Enzyme-Linked Immunosorbent Assay

Real confirmation of potentially deadly samples

ELISA is a biochemical technique used to detect the presence of an antibody or an antigen in a sample. In simple terms, an antigen is affixed to a surface and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal.

Brukers pTD uses a sandwich ELISA procedure for the detection of toxins. Antibodies immobilised on gold electrodes attached to a toxin chipstick facilitate the specific capture of corresponding toxins from an applied liquid sample. The detection of the captive toxins is realized by measuring the electrical current of an enzymatic redox reaction. The current correlates to the amount of target bound to the specific antibodies. 5 toxins can be rapidly detected in parallel.