Proteins are implicated in all living organism processes. Their complex structure, interactions dynamics in concentration, degradation or modification make the study of their actions and relationships a considerable challenge. Proteomics is about identifying, quantifying and characterizing the protein content of an organism, an organ, a subcellular fraction or even of a more purified subset of proteins. The final goal of proteomics is to help decipher the puzzle of life to gain efficiency in the design of the next-generation cures, diagnosis tools or biotechnological advances.