MALDI Biotyper® for Food Microbiology

AOAC and ISO/DIS 16140-part 6 validated


The MALDI Biotyper® system (MBT) provides specific and reliable identification of microorganisms within minutes. It’s recent validation and certification according to the Official Method of Analysis program (OMA) of the AOAC International and according to the new ISO/DIS 16140-part 6 standard, offers now a reliable one system, one workflow solution for food microbiology, in terms of safety and quality.

Certification / Validation

ISO/DIS 16140-6:2017
The MBT has been validated as a complete food microbiology solution for confirmation of:
 
Cronobacter spp.      Certificate N2017LR72
Salmonella spp.        Certificate N2017LR73
Campylobacter spp.   Certificate N2017LR74
Listeria spp. and        Certificate N2017LR75
Listeria monocytogenes,

in accordance with ISO/DIS 16140-6:2017 and the MicroVal Rules and Certification Scheme version 8.
The certificates are available on www.microval.org.

AOAC-OMA
The MBT has been certified according to the Official Method of Analysis program (OMA) of the AOAC International, for confirmation and identification of:

Salmonella spp.
Cronobacter spp.
and other gram-negative bacteria
First Action AOAC Official Method 2017.09

Listeria spp.
Listeria monocytogenes
and other gram-positive bacteria
First Action AOAC Official MethodSM 2017.09


The OMA 2017.09 and 2017.10 protocols are available on http://www.eoma.aoac.org/.

Pathogen confirmation made easy

The MALDI Biotyper is a Rapid Microbiological Method for microorganism identification based on proteomic fingerprints, useful in food microbiology. Its workflow has been designed to be as robust and easy to perform as possible. Only a few simple steps are required to generate a high-quality, reliable microorganism confirmation/identification - within minutes after detection of a positive selective culture, and without a time-consuming purification step. These time savings can be directly translated into significant cost savings in terms of lost product shipments and production line uptime.

Broad coverage of relevant foodborne microorganisms

The annually updated MBT Compass® reference library currently comprises 7,854 reference spectra of 2,748 species from 471 bacteria and fungi genera, including microorganisms important for food and beverage safety and quality controls. A dedicated MBT Filamentous Fungi Library is available as well, expanding the identification capabilities to 152 species / species groups. The reference libraries are not only a reliable source for confirmation of food pathogens or quality indicators such as Salmonella spp., Listeria spp., L. monocytogenes, Campylobacter spp., or Cronobacter spp.; but also allow the quality control of starter cultures and other fermentation, ripening/maturation strains, and the identification of microbial spoilers such as species of Pseudomonadales, Enterobacteriaceae, spore forming bacteria, yeasts and molds.

Creating custom libraries

Quality control laboratories that want to create their own library entries can make use of the optional MBT Explorer® Software to compile customized in-house libraries. These might be libraries with site-specific contaminants and / or important species used for production such as probiotic cultures, lactic and acetic acid bacteria, fermentative yeast and ripening molds.  Customer-generated libraries can easily be exchanged, e.g. between laboratories in other plants of the same company.

Instant differentiation and characterization of high-risk microbial isolates

After a first identification to the genus or species level, the MBT Subtyping Module screens some characteristic peaks to improve the discrimination level. This deeper screening is automatically triggered after identification, no additional work is required.
The MBT Subtyping Module is very useful for several applications in food safety and food quality, such as differentiation of the closely related Listeria species for fast confirmation of L. monocytogenes. This is now as easy as it can be, due to implementation of the MBT Subtyping Module into the MALDI Biotyper workflow for food safety.
Also for the characterization of methicillin-resistant Staphylococcus aureus (MRSA) in livestock and raw milk, the MBT Subtyping Module is useful, by automated detection of MRSA isolates carrying the surface peptide PSM-mec.

One system – one workflow

Not only pathogen confirmation benefits from the easy MALDI Biotyper workflow. The same set of low cost consumables, the same system, the same software, the same workflow can be used for fast and easy microorganism identification, be it bacteria, yeasts or molds. More detailed information can be found in our brochure.

IAFP_Workflow.png
Selected by AOAC International as Method of the Year 2018

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For General Purpose Use (In the United States, Japan and Taiwan: For Research Use Only (RUO)).
This product has no declared clinical intended purpose and is not for clinical diagnostic use. Any clinical diagnostic use is at the user’s own risk and responsibility.