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Capturing free radicals with Electron Paramagnetic Resonance (EPR) Spectroscopy

This webinar took place on June 04th 2019

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Electron Paramagnetic Resonance (EPR) spectroscopy is a magnetic resonance technique that can detect free radicals and transition metal ions.

It is a unique technique for shedding light on radical reactions and processes because of its ability to detect unambiguously paramagnetic species in a direct and non-intrusive manner. It is very versatile because it identifies, quantifies and characterizes paramagnetic species. EPR can be applied to samples in gaseous, liquid or sold states over a wide range of temperatures.

EPR spectroscopy is used to:

  • Detect, identify, and quantify free radicals
  • Study molecular structures, geometry, and dynamics
  • Observe labeled species in situ in biological systems
  • Understand redox processes, reaction kinetics, catalysis and more


This webinar introduces the technical and practical aspects, and capabilities of EPR spectroscopy to detect free radicals and transition metals in various types of samples and chemical processes. In addition, examples and tips on how to achieve successful experiment will be given with how-to’ videos:

  • Following the kinetics of radical chemical reactions
  • Identifying and quantifying radical intermediates
  • Measuring metal complex
  • Monitoring photo degradation



What you will discover

What attendees will learn:
The potential of EPR in academic and industrial research will be highlighted along with:

  • How to monitor radical reactions
  • How to do quantitative EPR analysis using the SpinCount tool
  • How to identify EPR-active species in multicomponent signals providing concentrations of individual components via the SpinFit tool
  • A variety of examples from how to do sample preparation to data acquisition and analysis.

Who should attend?

Academics who do chemical research or/and collaborate with companies to study chemical processes, and researchers in chemical industry.


Dr. Kalina Ranguelova
Dr. Kalina Ranguelova
Senior EPR Applications Scientist, Bruker BioSpin
Dr. Kalina Ranguelova is an EPR Applications Scientist in Bruker BioSpin Corporation since 2011. She completed her Ph.D. at The Bulgarian Academy of Sciences where she received a Ph.D. with research focused on inorganic copper complexes structure using electron paramagnetic resonance (EPR) spectroscopy. After two research positions at CUNY and National Institute for Environmental Sciences where she studied free radical biology and EPR spin trapping as method for measurement of reactive oxygen species (ROS), she joined Bruker and holds a role as Applications Scientist. Her current focus is detection and identification of free radicals in biological systems and pharmaceuticals using spin traps and spin probes. She has publications in journals like Journal of Biological Chemistry, Biochemistry, Free radical Biology and Medicine, etc. She has presented in many international meetings related to free radical research in biology and protein chemistry.
Dr. Ralph Weber
Dr. Ralph Weber
EPR Applications Manager
Dr. Weber received his Ph.D. in chemistry from the University of Chicago. This was followed by postdoctoral positions at Leiden University (NL) working on ODMR and MIT working on DNP. He joined Bruker in 1989 as an application scientist and specializes in pulse and high frequency EPR and EPR imaging.