The LUXENDO MuVi SPIM PM is an extension of the MuVi SPIM capabilities to enable photomanipulation of your sample.
Whether you want to cut structures within cells and tissues (photoablation), photobleach fluorescence in selected regions of interest for FRAP or activate proteins for optogenetics, the MuVi SPIM PM module can be configured to meet your needs by choosing between three different types of lasers systems. It can be added to both MuVi SPIM LS and CS (photomanipulation only possible when LS octagon mounted).
The MuVi SPIM PM illumination beam is coupled into the right-side detection objective lens, creating a diffraction-limited illumination spot. Its size is determined by the properties of the detection objective lens.
A 2D scanner allows to freely position the illumination spot in 3D in the sample while it is being imaged – fully integrated in the acquisition software. Complex illumination regions (point, straight and freeform line, square) are either part of the experimental workflow or can be defined interactively on the fly while a 3D imaging experiment is carried out.
For different applications, the MuVi SPIM PM can be configured with different types of lasers, ranging from CW UV to pulsed IR lasers.