The gap between LC-separation and MALDI-TOF analysis is securely bridged with our spotOn system for target preparation and fraction collection. With guided control from vial to target spot, intuitive workflows, and full visibility into both separation and preparation, potentially complex bottom-up and top- down analyses of biologicals are met with confidence and ease.
Complex biological samples, including industrially-produced biopharmaceutical products, often require chromatographic separation for in-depth analysis, as they include a very wide and dynamic range of components. Structural features critical to therapeutic function may represent only a small percentage of the total protein content, and even small impurities may necessitate immediate action within a production scheme. The hardware and software components of our spotOn system are designed to support, accelerate, and improve these analytical workflows, saving time and money and enabling a more comprehensive insight into these valuable biologicals.
The Bruker spotOn system is easily connected to a nano- or capillary-LC system and applies LC fractions to our reusable 384 or 1536 spot MALDI targets. Eluent application may be made by fraction time (volume) or the number of fractions desired, and near neighbor calibrants may be included in the target preparation for increased mass accuracy. MALDI matrices can be applied concurrently with the aliquots, or after a controlled drying time, resulting in uniform target spots ready for MALDI analysis. Whether using our nanoElute LC system or instrumentation from other vendors, fractions with analytical LC runs, can simply be collected in well plates for subsequent analyses.
For improved reproducibility and data quality, our spotOn system is built with double independent concentric needles (DICNTM), with an outer needle for piercing seals and septa, and a spotting needle that moves inside the outer needle and dispenses samples. Protected within the outer needle, the spotting needle can be optimized for volume and inertness. The needle pair is thoroughly cleaned inside and out with a combination of air pressure and solvent streams via the spotOn’s PowerWashTMsystem, preventing carryover.