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采用 Efree 探头进行的实验

生物固体及材料的专用高端技术

许多蛋白质没法使用 X 射线晶体学或液态 NMR 来处理,因为:

  • 尤其是膜蛋白

  • 溶解性差或无法溶解

  • 无法结晶或结晶化不够,无法使用 X 射线

越来越多的群体参与到让解决方案策略适应固体的快速方法开发中。与此同时,客户对此方法越来越关注,使得 Bruker BioSpin 脉冲项目库拥有了多种成熟的方法和脉冲项目。让新晋研究人员也得以进入生物固体 NMR 领域。

700 MHz HCN CPMAS

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GB1 project on Efree 4 mm 700 MHz HCN CPMAS probe. Experiments performed at and above room temperature. Resolution as found through isoleucine resonances better than 15 Hz. Sample courtesy of Vladimir Ladhizansky (U. Guelph).
700 MHz HCN CPMAS
GB1 project on Efree 4 mm 700 MHz HCN CPMAS probe. Experiments performed at and above room temperature. Resolution as found through isoleucine resonances better than 15 Hz. Sample courtesy of Vladimir Ladhizansky (U. Guelph).

700 MHz HCN CPMAS

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GB1 project on Efree 4 mm 700 MHz HCN CPMAS probe. Experiments performed at and above room temperature. Resolution as found through isoleucine resonances better than 15 Hz. Sample courtesy of Vladimir Ladhizansky (U. Guelph).
700 MHz HCN CPMAS
GB1 project on Efree 4 mm 700 MHz HCN CPMAS probe. Experiments performed at and above room temperature. Resolution as found through isoleucine resonances better than 15 Hz. Sample courtesy of Vladimir Ladhizansky (U. Guelph).

静止

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750 MHz Efree SAMMY spectrum of 2 mM <sup>15</sup>N-uniformly labeled Vpu in 14-O-PC/6-O-PC bicelles. Spectrum courtesy of S. Opella, UCSD.
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700 MHz Efree PISEMA spectrum of KdpF: 30 residue protein from the Mycobacterium tuberculosis genome, in oriented membranes. Sample courtesy of T. Cross NHMFL.