Pixel resolution: The top 3 images show triangular-shaped DNA origami imaged in buffer solution using a typical pixel resolution of 512 pixels. Other high-speed AFMs often reduce the number of pixels in an image to 100 or less to achieve higher scan rates, thereby requiring very small scan sizes to minimize the loss of resolution due to larger pixel sizes. There is a lower limit to the number of pixels used to acquire an image before the features of interest in your image can no longer be viewed.
Imaging speed: Traditional AFM images of DNA origami obtained at this high of a pixel density are typically conducted at 1 or 2 Hz, which results in each image taking several minutes to capture. FastScan-Bio clearly shows the individual DNA strands that make up each piece of origami, as well as the single stranded DNA end piece observed on the side of at least 2 of the triangles, at scan rates of 11Hz to 43Hz without loss of resolution.
Origami DNA in buffer: (350nm x 350nm x 3.4nm). Acquisition times at the given frequencies were: 11Hz = 46sec, 22Hz = 23sec, 43Hz = 12sec, 49Hz = 5sec, 87Hz = 3sec, 98Hz = 2.5sec. Sample courtesy of P. Rothemond & L. Qian, CalTech, USA.