Fluorescence Microscopy combined with nanomechanical testing provides the ability to accurately position nanomechanical tests on specific regions of material marked with fluorochromes. Common brightfield microscopy, standard on most nanomechanical test instruments, utilizes broad-spectrum reflected light to image the sample surface and cannot readily distinguish testing regions of interest on complex inhomogeneous materials. By allowing researchers to selectively tag individual constituents within the material, accurate and reliable test positioning can be achieved.
Bruker has developed a fully integrated fluorescence microscope for the Hysitron TI 980 TriboIndenter and Hysitron TI 950 TriboIndenter nanomechanical test instruments. Nanoindentation and fluorescence imaging is combined utilizing a high-precision automated staging system and provides exceptional spatial synchronicity between the techniques. The fluorescence microscope utilizes user-definable filter sets for use with a wide range of fluorescent dyes and is also capable of brightfield imaging, allowing the nanoindenter system to be effectively utilized in a variety of research areas.