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质谱网络讲座 – 在线及往期回顾

Bruker网络讲座 了解最新的技术,科技发展以及创新的分析解决方案,并获取最新信息。




10 am (GMT+8)

Presenters:林诚, 副教授, 波士顿大学医学院
多糖在结构上的复杂性和多样性,对应了其在生物体内的不同功能。与一级结构由一维序列所决定的线性生物分子(例如蛋白质及核酸)不同,多糖的详细表征需要测定其几何状态和空间关系、连接位点及立体化学构型。进一步的分析挑战来自于多糖生物合成的“非模板驱动”特点,导致其糖组(glycomes)包含了一系列结构相似的多糖,其中大部分是异构体。由于潜在的多糖结构数目庞大,加上缺乏综合的多糖数据库,因而对多糖的测序是极大的挑战。目前多糖的结构分析也结合了串联质谱技术,尤其是电子活化裂解(electron activated dissociation,ExD)已经显示了对多糖,包括N-糖、O-糖、粘多糖等进行详细结构表征的潜力。本次讲座将会讨论用于多糖测序的ExD技术进展,包括ExD技术和液相色谱与离子淌度质谱的联用分离技术。

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4 pm (CEST)
Target and non-target metabolomics in environmental research; developmental neurotoxicity of biocides

Presenter: Prof. Pim Leonards, Institute for Environmental Studies, VU University of Amsterdam, Netherlands

Worldwide, serious concern has arisen about the increased incidence of learning and developmental disorders in children. Various recent epidemiological studies have indicated that exposure to low doses of environmental biologically active contaminants, such as biocides, during human development can have deleterious effects on cognitive development in childhood. In the EU-funded project DENAMIC we investigated neurotoxic effects (e.g. learning and developmental disorders) of low-concentration mixtures of biocides and a number of common environmental pollutants in children. An important aspect was to elucidate the mechanism of toxicity behind the (developmental) neurotoxicity using animal models (rat) based on (epi-)genomic, proteomic and metabolomic analysis. In this webinar we discuss the workflow to perform target and non-target metabolomics, and provide some examples to understand the underlying molecular mechanisms of observed effects of biocide exposure in rats in relation to behaviour and cognitive changes. For these studies LC-HRTOF-MS was used in combination with different software packages to map the biochemical networks affected. One of the interesting findings was that effects were sex specific and part of these differences could be explained by metabolomics.

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4 pm (CEST)
Hydrogen Exchange Mass Spectrometry automation for the maXis Ultrahigh Resolution QTOF

Presenters: Prof. Dr. Matthias P. Mayer, Center for Molecular Biology (ZMBH), University of Heidelberg

Hydrogen exchange mass spectrometry (HX-MS) is a highly suitable method to analyze the conformational state of proteins and changes caused by folding and unfolding, protein-protein-interactions, ligand binding and allostery. It can thus be used to assess the quality of protein preparations, to identify stable and flexible regions within proteins and how they change over time or under different conditions and for epitope-mapping. This webinar will cover principles of HX-MS and some of the basic considerations when setting up HX-MS experiments, and then discuss a fully automated system consisting of a LEAP robot and a Bruker maXis mass spectrometer.

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