Multiphoton Microscope

Ultima 2Pplus

A complete all-optical multiphoton workstation for imaging and optical manipulations
Ultima 2Pplus

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Ultima 2Pplus

With new advances in field of view, sensitivity, wavelength, and sample accommodation, the Ultima 2Pplus delivers an ideal combination of flexibility, resolution, imaging depth and speed, allowing users to perform simultaneous imaging, stimulation and electrophysiology protocols with greater efficiency and effectivity. The system is designed specifically for intravital imaging, with fully motorized control of the objective X-Y-Z position, as well as two axes of rotation for precise imaging orientation. A second scan path enables simultaneous imaging and photoactivation. The system’s optimized optical train confers exceptional performance to the very edges of the large field.

Class-leading
field of view
Offers a more complete picture with every scan.
High-efficiency
light collection and detection
Images deeper with a greater signal-to-noise ratio.
Simultaneous
imaging and photoactivation
Records cells with exact timing and spatial precision.

Características

Features

Largest Field of View and Superior Field Uniformity

The Ultima 2Pplus field of view is over 50% larger to facilitate modern optogenetics experiments, which require simultaneous imaging and photostimulation of distant regions in a sample. A new, custom optical design preserves spatial and temporal resolution throughout the entire field, which is essential for researchers to be able to continue their work without changing experimental designs.

Field of view on Ultima 2Pplus is FN 28 mm in comparison to industry standard of FN 18 mm.

High-Sensitivity Deep Imaging

1.3mm deep, 3D-view of pyramidal cells in layer 5. Data collected in one-month-old transgenic Thy1-YFP-H line mouse through a glass-covered cranial window. Data courtesy of Guang Yang, School of Medicine, New York University, New York.

The Ultima 2Pplus light collection path ensures detection of almost all photons scattered from dim, thick and opaque biological samples. The superior collection efficiency is provided with 2-inch or larger optics
throughout the emission path up to the sensitive close-proximity detectors. As two-photon microscopy has become well adopted, emerging three-photon microscopy offers the potential to image even deeper into
samples. Since further infrared wavelengths are scattered even less, three-photon excitation has the promise to be able to image several millimeters into the brain. Ultima 2Pplus already uniquely incorporates this capability to take your research to new levels of potential.

Perfected Photostimulation and Imaging

The Ultima 2Pplus empowers sophisticated optogenetics experiments with Bruker's Neuralight 3DTM Spatial Light Modulator option. Large populations of targets can be stimulated simultaneously in multiple focal planes with computer-generated holograms. During 3D holographic experiments, targets at different depths are imaged with a custom-designed, optically-corrected ETL focusing module that allows for independent Z-positioning of the imaging plane relative to photostimulation.

Optical stimulation of circled cells with Neuralight 3D. 3D hologram of points was created which was then spirally scanned over neuronal cell bodies. Data Courtesy of Adam Packer, Lloyd Russell, Henry Dalgleish, Michael Häusser’s lab, UCL, London.

Aplicaciones

Especificaciones

Specifications

SCAN HEAD
Scanning Method Matched pair of 6 mm Cambridge galvanometers with raster and spiral scanning capabilities
Field of View ~1.404 mm x 1.404 mm with 16x objective (up to 28.3 mm FN)
Scan Speed Raster scan: 1.65 fps at 512 x 512, >12 fps at 64 x 64
Spiral scan: 6 fps at 512 x 512, ~30 fps at 64 x 64
Scan Customization User-definable straight, freehand and circular (infinite) linescan with included software; user-definable pixels per line and lines per scan from 1 - 2048; up to 128x scan zoom; 360° of scan rotation; point scan
Uncaging Option Second set of matched 3 mm or 6 mm Cambridge galvanometers in the same scan head to provide high-precision visible or multiphoton laser sample photomanipulation
High-Speed Imaging Option 8 kHz resonant galvanometer; 30 fps at 512 x 512, >1300 fps at 512 x 8 region of interest
DETECTORS
Reflected Non-Descanned 1 - 4 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs
Transmitted Non-Descanned 1 - 2 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs
Dodt Single Hamamatsu PMT for DIC-like image collection
Transmitted Single Hamamatsu PMT for transmitted light image collection
Camera Standard C-mount camera port built into scan head
OPTICAL INPUTS
Multiphoton Laser Optimized for multiphoton laser input from 690 - 1700 nm
Epifluorescence High-Powered LED in epifluorescence turret
Visible Laser Fibered laser inputs for visible laser introduction
Ultima Laser Rating Class 1 (contains Class 3b and Class 4 lasers) with light box
Helios Laser Rating Class 3b
LED Full-field photoactivation with LED module
MOTOR CONTROL
Bruker X, Y Stage Variable height with ~15 cm X and ~7.5 cm Y movement and ~0.3 μm step size
Bruker X, Y Microscope Base Fine and coarse-movement platform for scope with ~35 mm travel and 0.1 μm step size
Bruker Z-Focus Range of 30 mm with ~0.2 μm step size
Bruker Z-Piezo Travel range of up to 1000 μm with 0.05 μm step size
Bruker Orbital Nosepiece Motorized control of objective angle and rotation
SOFTWARE
Prairie View Imaging Software fully integrated with scan head for easy imaging; customizable scan settings for optimization of specimen excitation; integrated control of laser power and PMT high voltages
Z-Series Easy creation of depth stacks with user-customizable slice number, step size and laser power
T-Series Easy creation of complex series involving Z-Series and triggered images
Stage Montage Atlas Imaging simplifies setup and optimizes acquisition of 2D and 3D stage montages
Peripherals Integration Wavelength and power control available for multiphoton and visible laser launches
Photoactivation User-defined points and regions with synchronized laser modulation
Regions of Interest User-defined regions for faster scanning capabilities
Brightness Over Time Intensity mapping and plotting for user-defined regions over time
Voltage Inputs/Outputs Signal inputs and outputs for electrophysiological experiments, stimulus control, and synchronization with external devices

Webinars

Testimonios

Testimonials

Hear What Our Customers Have to Say

We appreciate the modular nature of the Ultima hardware and software. The systems now available range from basic imaging systems to the dual-laser photo-stimulation multi-mode imaging workstation. We have continued updating many of our 14 machines, including our ten year old systems. The Team Viewer connection and support is a huge asset for novice owners and for significant software changes.

David Wokosin, Ph.D., Northwestern University

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