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rapifleXTM MALDI TissuetyperTM

rapifleX MALDI Tissuetyper closeup

Speed | Robustness | Definition

The rapifleX instrument re-defines the key performance measures for MALDI Imaging:

  • Up to 50 true pixels / second for faster and better images
  • smartbeam™ 3D laser for better pixel-to-pixel reproducibility
  • Pixel size < 10μm for highest spatial resolution to retrieve biological information
  • Novel ion source design for increased robustness

Laser and Ion Source Designed for MALDI Tissue typing - smartbeam 3D laser

During the image acquisition the sample stage moves continually to achieve high pixel rates. The newly developed smartbeam™ 3D laser fires at a repetition rate of up to 10 kHz.

 

The new smartbeam™ 3D laser features a laser diameter of 5um. As illustrated below, the laser can move independently from the sample stage to scan the full area of each pixel. This produces truly square pixels which utilizes all of the available sample area for maximum sensitivity and pixel-to-pixel reproducibility.

Ablated matrix showing square pixels
Ablated matrix showing square pixels.
Smartbeam laser movement
Dual rotating mirrors raster the laser beam in an x-y axis which is independent of the x-y motion of the target. The two independent movements combine to create the ideal sampling environment for Imaging: the true, welldefined pixels, as seen above, acquired at maximum acquisition rate.

rapifleX MALDI Tissuetyper - Designed for MALDI Imaging

  • MALDI time-of-flight system
  • New ion source for robust day-to-day operation
  • Novel smartbeam™ 3D laser
  • Fast acquisition of up to 50 true square pixel / second
  • Laser repetition rate 10 kHz
  • Pixel size <10μm

Integrated software solutions

  • flexImaging dedicated molecular histology software controls data acquisition, data visualization, annotation and integration of virtual microscopic slides
  • SCiLS Lab is the dedicated software for statistical analysis. From individual imaging datasets to complete clinical biomarker studies, it features univariate and multivariate methods to mine the data and to find relevant information.
Proteins in gastric cancer
Proteins in gastric cancer, pixel size 30 μm, 99000 pixels, 500 laser shots per pixel, measurement time 82 minutes.
Overlay of MALDI Tissuetyper image with microscopic image acquired from the same tissue section provides a clear link to histology
Overlay of MALDI Tissuetyper image with microscopic image acquired from the same tissue section provides a clear link to histology.
Related Information