Xiang Gao et al. conducted a study on various B. subtilis biofilms to understand bacterial response to transient physical stress and to determine the influence of calcium dynamics on mechanical properties, particularly stiffness.
Nanoindentation was performed using a Hysitron BioSoft in-situ indenter (Bruker, Minneapolis, MN) mounted on a Leica SP5 confocal microscope. The cocultured biofilms (with Si nanowires) were stimulated using the confocal microscope, and a laser (592 nm) was used to scan a region of interest of 300×300 µm2. The stiffness of the biofilm on structured silicon was measured at different locations with and without intercellular calcium signaling. A Hertzian contact model of a rigid sphere indenting into an elastic half space was used to analyze nanoindentation results. The laser-stimulated part of biofilm exhibited a reduction in stiffness (reduced by 20.8%) compared to unstimulated ones due to modulus alteration by coupling between calcium and biofilm.
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FEATURED BRUKER TECHNOLOGY:
Biofilms, Calcium Signalling, Microbial Biofilms, Signal Transduction, Silicon, Si Nanowires, Synthetic Biology