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Luxendo Light-Sheet Microscopes

InVi SPIM Lattice Pro

The inverted view light-sheet microscope with advanced illumination unit
InVi SPIM Lattice Pro

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InVi SPIM Lattice Pro

The InVi SPIM Lattice Pro provides the highest level of flexibility for illuminating samples using a proprietary Advanced Illumination Module (AIM). It expands the capabilities of the InVi SPIM: while maintaining the ease-of-use and stability of the system, it adds tailorable, interactive adaptability of the beam shape to suit the highly specific requirements of your sample. You choose what gives you the best results for your 3D high resolution imaging experiment – large field of view, high speed or optimal spatial resolution.

Características

Illumination and Detection

The InVi SPIM Lattice Pro offers a variety of illumination patterns, ranging from the classical static Gaussian light-sheet or a scanned Gaussian beam to sophisticated illumination schemes like Bessel beams, Airy beams or optical lattices.

The user can select from a broad choice of beam shapes to improve the microscope’s resolution and reduce photo-damage in delicate samples. A Special Optics 28.6x 0.7 NA water immersion objective lens projects the light-sheet on the sample. A high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens images the signal onto one or two Hamamatsu sCMOS cameras. An additional magnification changer provides 31.3x and 62.5x total magnification to allow you to optimize field of view and pixel size to your experimental needs.

InVi SPIM Lattice Pro Design

Not available in Germany.

Lux DATA - A Comprehensive Data Processing and Storage Solution

Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.

Aplicaciones

Live Sample Applications

Browse a selection of applications data from our customers below. Researchers are using the InVi SPIM Lattice Pro in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.

Mitosis in HeLa Cells

Mitosis in HeLa cells stained for histone 2B-mCherry (magenta), GFP-tubulin (green) and GFP-tubulin (white, deconvolved).

Imaged on the InVi SPIM Lattice Pro.

Visualization: Imaris (Bitplane).

Courtesy of:
Sabine Reither
European Molecualr Biology Laboratory (EMBL)
Heidelberg, Germany

Sample: HeLa cells (Neumann et al., Nature. 2010 Apr 1;464(7289):721-7)

3D Imaging of a Spheroid

Spheroid labeled with EGFP and mRFP imaged on the InVi SPIM Lattice Pro. Three illumination patterns were tested for each label: Gaussian beams, Bessel beams, and optical lattices. The optical lattices gave the best results for the EGFP labeling, while the Gaussian beam was optimal for the mRFP labeling.

Courtesy of:
Martin Stöckl
University of Konstanz
Germany

HeLa Cell Culture

HeLa cells expressing GFP and mCherry. Imaged on the InVi SPIM.

Courtesy of:
Tobias A. Knoch
Erasmus MC
Rotterdam, The Netherlands

Especificaciones

Specifications

Illumination Detection Effective Magnification Field of View Pixel Size Optical Resolution
28.6x / 0.7 NA Nikon 25x / 1.1 NA

31.3x

62.5x

420 µm

210 µm

208 nm

104 nm

255 nm

Illumination Optics

  • Chromatic correction from 440 to 660 nm
  • Light-sheet generation with static illumination or by beam scanning
  • Flexible light-sheet geometries: static and scanned Gaussian beams, Bessel and Airy beams and optical lattices for improved resolution, field of view and speed
  • Special Optics 28.6x 0.7 NA water immersion objective lens

Detection Optics

  • Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
  • 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
  • Filters adapted to the selected laser lines
  • 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
  • Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
  • Peak quantum efficiency (QE): 82% @ 560 nm

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