The detection principle of the pBDi is based on the well established ELISA procedure combined with an electrochemical readout.

Capture antibodies immobilized on gold electrodes facilitate the specific binding of corresponding biothreat agents. Detection of bound biothreat agents is realized by application of a detector-antibody-enzyme conjugate and measurement of the electrical current of an enzymatic redox reaction.

The electric signal is strongly amplified in this system and allows very sensitive biothreat agent detection in approx. 20 minutes. First, the high turnover of enzymatic reaction contributes to the signal amplification and second, a redox cycling procedure built into the experimental procedure, provides an additional signal amplification. The straightforward workflow starts with resuspension of a liquid or solid sample in a supplied sample buffer.

pBDi Features

  • Broad application range: Universal and reliable detection of up to 6 toxins, bacteria and viruses in parallel assayed in duplicates along with internal assay controls
  • Easy-to-use ELISA-based technology: Special cartridge and reagent holder design enable safe handling by unskilled operators.
  •  Automated sample processing and data evaluation: Wizard-based control software with fully-automated data processing and “traffic light” based result display.
Principle of electrochemical immunoassay

Principle of electrochemical immunoassay

  • Outstanding test time combined with high sensitivity: Receive test results in about 20 mins with sensitivities down to pg/ml range for toxins; 10³ CFU/ml for bacteria and 104 PFU/ml for viruses.
  •  Portability and possibility to decontaminate: Battery-based operation modus and suitcase integration enable field and mobile applications by first responders.
  •  Freeze dried ready-to-use kits: Enable long-time storage up to 12 months and reduce ownership costs.

  •  Open array concept: Self-immobilization protocols enabling users to create their own assays.