Toxtyper

Toxtyper®: a complete solution for clinical research and forensic toxicology

Today's forensic toxicology and clinical research labs are challenged by demands for fast and accurate results, along with lab efficiency and cost reduction. Further pressure results from the continuous emergence of new drugs into the market, requiring frequent updates of sample screening methods.

The Toxtyper® LC-MSⁿ approach with library searching is an easy to use, flexible, and reliable screening solution for modern toxicology research.

The Toxtyper® holds a curated high quality spectral library with approximately 3000 MSⁿ spectra of nearly 1200 compounds of research toxicology interest, and a smaller targeted library for drugs of abuse (DOA) screening research. Additionally, the Toxtyper® supports the Maurer/Wissenbach/Weber LC-MSⁿ library* containing almost 6000 drugs and metabolites.

^{*Maurer/Wissenbach/Weber LC-MSn Library of Drugs,  Poisons and Their Metabolites 2nd Edition; ISBN: 978-3-527-34649}

From comprehensive screening to semi-quantification

Different well-established sample preparation methods, such as liquid-liquid extraction (LLE), solid-phase extraction (SPE) or simple protein precipitation, can be used within the Toxtyper® workflow. Protein precipitation is quick and easy and is well suited for the comprehensive analysis of a wide range of analytes.

Example data demonstrating simultaneous, highly sensitive detection of drugs in human urine. Following simple sample preparation (liquid-liquid-extraction) of 1 mL of urine, the Toxtyper® permits detection down to 0.8 ng/ml.

Although drug analysis using immunological methods is fast, it is not always reliable. The available screening tests can easily lead to false positive results. For the confirmation of such immunoassay results,
complex and time-consuming GC-MS analysis has been so far performed by the Stuttgart hospital.

Since March 2017, the Toxtyper® system has been used for the screening of drugs and toxins in blood, oral fluid and urine. Without time-consuming sample preparation, Toxtyper® delivers fast and reliable results that are highly specific and reproducible. In addtion, the Toxtyper® also provides a high degree of safety in toxicological analysis prior to the neurological diagnosis of irreversible brain failure.

Case Study

A 34 year old patient with dizziness, nausea and vomiting is admitted to the emergency room.From the anamnesis there is a suspicion of drug consumption, however the patient denies the use of illegal drugs. Urine screening using various immunoassays was negative for Amphetamines, Barbiturates, Benzodiapezepins, Cocaine, Opiates and Cannabis. The Toxtyper® identified Mitragynin and several metabolites of Paynanthein and Speciociliantin, which are alkaloids present in leaves and fruits of the Kratom tree. Kratom is used for substitution of Opioids and is not regulated by the German narcotics law.