Product banner timsTOF flex

Combining the world’s fastest proteomics instrument with over 25 years of MALDI experience

Match PASEF powered LC-MS/MS identification with label-free spatial localization to decode the molecular make up of your sample

Built on the standard for shotgun proteomics, the timsTOF fleX combines best in class 4D X-Omics with Bruker’s cutting edge MALDI Imaging technology, including SmartBeam 3D laser optics for fast measurement all in one platform. A dual source instrument ideal for SpatialOMx, timsTOF fleX conducts robust ESI measurements and spatially resolves a wide range of molecules directly from tissue using one platform. No single instrument has before provided access to both essential capabilities for the most advanced OMICS researchers. 

Schematic of the timsTOF fleX
Schematic of the timsTOF fleX

From 4D OMICS to molecular imaging without stopping, changing, modifying or compromises

SpatialOMx demands PASEF enabled LC-MS/MS for deep 4D OMICS combined with the ability to create molecular images of tissue sections with MALDI Imaging. To do this under one platform, Bruker engineers started with the award winning TIMS dual-stage ion funnel and incorporated target stage and laser elements from the rapifleX axial TOF instrument.  During operation, software activation of the SmartBeam 3D laser is the only change in the source region.  No complicated changeover means making zero compromises in productivity and the ability to move effortlessly from world class OMICS identification and quantification workflows to creating high definition molecular maps of tissue sections to see what matters most. 

Since ions produced by MALDI and ESI travel the same path to the detector from the source, MALDI workflows can take advantage of the most advanced features found in the timsTOF Pro, including TIMS ion mobility separation based on the collisional cross section of detected molecules and high-speed high-sensitivity PASEF fragmentation. Tuning and calibration can be performed in ESI mode and used for the MALDI experiment for additional ease of optimization.

Direct molecular profiling from tissue with MALDI Imaging

MALDI Imaging is a label-free tool for examining the distribution of molecules directly from thin tissue sections. Applicable for examining a wide range of analytes, including metabolites, lipids or glycans, and seamlessly compatible with microscopy workflows, MALDI Imaging can identify regionally specific compound distributions for SpatialOMx analyses. Use Bruker’s IntelliSlides for automated MALDI Imaging and SpatialOMx workflows on the timsTOF flex.

TIMS removes isobaric interferences in lipid imaging
TIMS removes isobaric interferences in lipid imaging.
Blue: m/z 762.6 +/-0.016; mob. 1.45 +/- 0.012
Yellow: m/z 762.6 +/-0.016; mob. 1.48 +/- 0.015

Zoom mode to see what really matters

Besides the ability to generate high definition “True Pixel” MALDI images, the SmartBeam 3D laser source provides rock solid laser guided targeting down to 10 µM with Bruker’s new zoom mode technology. Users now have the flexibility to adjust their laser spot size and spacing to accommodate a wider range of granularity depending on biological features of interest.

Mouse retina
Mouse retina measured for lipids at 10 µm in Zoom mode. Scale bar indicates 100 µm
(Image courtesy Jeffrey Spraggins, Ph.D., Mass Spectrometry Research Center, Vanderbilt University)

For Research Use Only. Not for Use in Clinical Diagnostic Procedures.