Force-Distance Measurements

Targeted characterization of individual force interactions

The binding force with which a ligand binds to a receptor molecule often has profound downstream effects on a biological system. Such interactions are important in cell signaling and pharmaceutical research, among other areas. Force-distance measurements enable researchers to study attractive and repulsive forces on a probe as it approaches and retracts from a sample surface. Additional data can be obtained by attaching molecules of interest to the probe tip.

Force-distance techniques are commonly applied to investigating fundamental force interactions, nano-scale adhesive and elastic response, binding forces, colloidal studies, and chemical sensing. Force-distance is just one technique in Bruker’s comprehensive Nanomechanics Package, along with PeakForce QNM™ and quantitative Force-Volume mapping.

Featured on the following Bruker AFMs:

Recommended AFM probes:

For biological specimens:

For non-biological specimens:

HeLa cells express varying amounts of fluorescence-tagged membrane protein. Force measurements performed with functionalized AFM probes show a strong correlation between binding force and fluorescence intensity, with bright cells yielding many binding eve