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Application Notes - Magnetic Resonance

Mouse Imaging

With the help of the mouse diseases, such as cancer, cardiovascular diseases or brain disorders, can be researched.
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The mouse and humans share a common ancestor, thus it is of no surprise, that their genes and genomes are very similar. Due to this similarity, the mouse is used as a model organism to study human biology. With the help of the mouse diseases, such as cancer, cardiovascular diseases or brain disorders, can be researched.

MR microscopy enables to acquire in-vivo images of the neuronal pathways in the brain (Figure 2& Figure 3) and even record videos of a beating heart (Figure 3), where each single image of the video sequence can be triggered by EKG, for better image resolution. Additional marker substances enable highlighting area such as the inflammations in Figure 4.

High-resolution gradient echo experiment on an ex vivo mouse brain. : 3D FLASH, (15 x 15 x 25) µm3, the coil was MICWB40 RES 700 1H/13C, TR/TE 100/11 ms, AV=2, flip =60°, BW 89285 Hz, 1.37 PartialFourier in 1st PE direction, Total scan time = 15h 14min
Visualization of all four heart chambers and aorta. IntraGateFLASH, TR: 10 ms, TE: 1.8 ms, resolution: (78 × 59 × 800) µm³, scan time: 8 min
Diffusion-weighted magnetic resonance, 16.4 T vertical bore, 15 mm linear coil, TR/TE 400/22.8 ms; resolution 0.1×0.1×0.1 mm, b=5000 s/mm2, δ/Δ=2.5/14 ms, total imaging time 32 h.
MSME 1H reference image (FOV 40 x 40 mm2, 0.16 x 0.16 x 1 mm3 ) next to a RARE perfluorocarbon labeled 19F image (FOV 40 x 40 mm2, 0.31 x 0.31 x 1 mm3) and the combined image. The images were acquired with a 30 mm 1H/19F Birdcage resonator. Perfluorocarbon labeled macrophage and monocytes travel to the places of inflammation, leading to a local accumulation of these cells. These accumulations can be visualized by 19F magnetic resonance imaging.
MSME 1H reference image (FOV 40 x 40 mm2, 0.16 x 0.16 x 1 mm3 ) next to a RARE perfluorocarbon labeled 19F image (FOV 40 x 40 mm2, 0.31 x 0.31 x 1 mm3) and the combined image. The images were acquired with a 30 mm 1H/19F Birdcage resonator. Perfluorocarbon labeled macrophage and monocytes travel to the places of inflammation, leading to a local accumulation of these cells. These accumulations can be visualized by 19F magnetic resonance imaging.
MSME 1H reference image (FOV 40 x 40 mm2, 0.16 x 0.16 x 1 mm3 ) next to a RARE perfluorocarbon labeled 19F image (FOV 40 x 40 mm2, 0.31 x 0.31 x 1 mm3) and the combined image. The images were acquired with a 30 mm 1H/19F Birdcage resonator. Perfluorocarbon labeled macrophage and monocytes travel to the places of inflammation, leading to a local accumulation of these cells. These accumulations can be visualized by 19F magnetic resonance imaging.

References: 

Bruker Application note: Cardiovascular Magnetic Resonance in Mice, Ulrich Flögel, Experimental Cardiovascular Imaging Heinrich Heine University, Düsseldorf, Germany floegel@uni-duesseldorf.de, 

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwiu0OX2mNbpAhXnUBUIHQarClUQFjABegQIAxAB&url=https%3A%2F%2Fwww.bruker.com%2Ffileadmin%2Fuser_upload%2F8-PDF-Docs%2FPreclinicalImaging%2FT177101_App_Note_Cardiovascular_Magnetic_Resonance_in_Mice.pdf&usg=AOvVaw3wGL-QsA312ORCKC9Ho5qp (better link needed)

Ianuş, Andrada, et al. "Accurate estimation of microscopic diffusion anisotropy and its time dependence in the mouse brain." Neuroimage 183 (2018): 934-949.

Moldrich, Randal X., et al. "Comparative mouse brain tractography of diffusion magnetic resonance imaging." Neuroimage 51.3 (2010): 1027-1036.