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Light-Sheet Microscopy: Seeing Life From a Different Angle

12:00 PM - 1:30 PM - November 12, 2019 | University of Washington, Seattle, WA

Invitation

The University of Washington and Bruker invite you to join us for a “Light-Sheet Microscopy Lunch and Learn” which will introduce the relatively new 3D imaging technique called light-sheet fluorescence microscopy (LSFM). LSFM is opening the door for many researchers to perform high-speed 3D imaging of large and delicate samples while still being able to observe fast subcellular processes and interactions within the organ, organoid or organism context. Lunch will be provided.

Attendees can expect to learn:

  • Basics of LSFM
  • Differences between LSFM, spinning disk confocal and laser scanning confocal microscopy
  • Advantages of LSFM for applications ranging from cleared samples to high-resolution cellular imaging
  • How LSFM can be adapted to a multitude of different samples

 
Should you have any questions, please feel free to contact Dane Maxfield, Ph.D., Western Region Sales Manager, at dane.maxfield@bruker.com.

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Event Location

University of Washington
Life Sciences Building, Room 401
W. Stevens Way, NE
Seattle, WA 98195

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Lunch and Learn Host

University of Washington

About Light-Sheet Fluorescence Microscopy

Light-sheet fluorescence microscopy (LSFM) is a state-of-the-art imaging method to address a wide variety of biological questions. By decoupling the illumination and detection optical pathways, LSFM allows for a novel illumination strategy that optimizes the photon detection efficiency by only illumining the sample plane of interest. Since fluorescence detection is preformed along a different optical axis, this typically requires two (or more) objectives in an orthogonal orientation in order to maximize detection and minimize fluorescence from out of focus light. This axial confinement of the illumination beam results in LSFM having an extremely low rate of photobleaching and phototoxicity, allowing for long-term imaging for hours, days and even weeks.