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Light-Sheet Microscopy: Seeing Life From a Different Angle

12:00 PM - 2:00 PM - February 26, 2020 | University of California, Los Angeles

Event registration has reached maximum capacity.

Invitation

The UCLA MCDB-BSCRC Microscopy Core and Bruker invite you to join us for a “Light-Sheet Microscopy Lunch and Learn” that will introduce the relatively new 3D imaging technique of light-sheet fluorescence microscopy (LSFM). LSFM is opening the door for many researchers to perform high-speed 3D imaging of large and delicate samples while still being able to observe fast subcellular processes and interactions within the organ, organoid or organism context. There is a maximum capacity of 50 people for the seminar room, lunch will be provided.

Attendees can expect to learn:

  • Basics of LSFM
  • Differences between LSFM, spinning disk confocal and laser scanning confocal microscopy
  • Advantages of LSFM for applications ranging from cleared samples to high-resolution cellular imaging
  • How LSFM can be adapted to a multitude of different samples

 
Please contact Dane Maxfield, Ph.D., Western Region Sales Manager (dane.maxfield@bruker.com) with any questions, or if you would like to learn more about Bruker's light-sheet microscopy solutions.

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Event Location

University of California, Los Angeles
Biomedical Sciences Research Building, Room 154
615 Charles E. Young Drive, South
Los Angeles, CA 90095

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Lunch and Learn Host


About Light-Sheet Fluorescence Microscopy

Light-sheet fluorescence microscopy (LSFM) is a state-of-the-art imaging method to address a wide variety of biological questions. By decoupling the illumination and detection optical pathways, LSFM allows for a novel illumination strategy that optimizes the photon detection efficiency by only illumining the sample plane of interest. Since fluorescence detection is performed along a different optical axis, this typically requires two (or more) objectives in an orthogonal orientation to maximize detection and minimize fluorescence from out-of-focus light. This axial confinement of the illumination beam results in LSFM having an extremely low rate of photobleaching and phototoxicity, allowing for long-term imaging for hours, days and even weeks.