Bruker at IMSC

Thank you for joining us at the 21st International Mass Spectrometry Conference

Thank you for your interest in Bruker’s scientific posters presented at the 21st International Mass Spectrometry Conference (IMSC) annual meeting, held August 21st through the 26th in Toronto, Canada. One registration is all you need to access any or all of this year’s posters. The availability of these posters is another example of Bruker’s ongoing commitment to helping scientists accelerate their research, achieve breakthroughs, and develop new Mass Spec related applications that improve the quality of human life. 

 

The first four posters highlight experiments performed using innovative new system technologies that Bruker introduced this year.

Bruker at IMSC 2016
Bruker at IMSC 2016
Imsc poster MALDI ft icr

Ultra high resolution mass spectrometry offers numerous advantages for targeted and discovery MALDI imaging analyses. Among the most cited of these is the resolution of isotopic fine structures and measurement accuracy which provide thousands of molecular images in a single dataset and can often yield unambiguous assignment of a single molecular formula to a detected peak. By their nature, however, Fourier-based instruments achieve high performance at a cost of larger spectral size and slower acquisition. MALDI imaging datasets are continually growing in the number of pixels or spectra as the technique is applied to more diverse samples and achievable spatial resolution improves. It is not uncommon for an image to contain100,000 spectra or more, often forcing one to compromise high MS performance for a higher acquisition rate. For example, at a rate of 1 s/spectrum a 7T FTICR can achieve a resolution of 300,000 at m/z 300. Acquiring a 100,000 pixel image at 1 s/spectrum will take >27 hrs. Quadrupolar detection, allows for the collection of the time-domain transient at 2x the cyclotron frequency, automatically doubling the analytical performance for the same transient length. For MALDI imaging the alternate implication has greater bearing, namely the same analytical performance can be achieved in half the transient  length, or half the acquisition time.

Imsc poster 2 omega ft icr

Technological innovations in mass spectrometry typically provide fractional improvements in critical analytical figures-of-merit for detector-based systems. Imagecurrent-based detection schemes (ICR, Orbitrap, etc.) yield substantially greater improvements as witnessed in recent advancements such as the absorption mode processing (AMP) that roughly doubled temporal resolving power. Due to recent innovations and advancements in frequency based detection technology, the concept of high performance mass spectrometry already shifted from a molecular based detection methodology to an atomic one based on resolved neutron discrimination, i.e. isotopic fine structure (IFS). Here we provide results of that reality using a unique combination of novel frequency-based technologies and the special considerations of the application of that capability to create novel and efficient workflows in analytical mass spectrometry.

Imsc poster ion mobility isomeric compounds

Ion mobility (IM) separation hyphenated with high resolution accurate MS extends substance identification and characterization capabilities to a new dimension.

IM permits to separate isobaric or isomeric compounds. In addition, it delivers a specific physical property of any compound: the collisional cross section (CCS) value. We applied Trapped Ion Mobility Separation (TIMS) on the new timsTOFTM instrument to evaluate its extended capabilities for compound separation and characterization. The model compounds were isomeric trisaccharides or lipids. In real-world samples these compounds are difficult to identify using LC-MS-only methods as these don´t provide clean MS/MS spectra. The Bruker timsTOF system with its ion mobility expansion technology (imeXTM) facilitated method development for an appropriate IMS separation.

Imsc poster ion mobility

Due to the immense structural diversity of flavonoids, the chemical analysis of complex samples such as natural bee resin that contain Propolis, is complicated (e.g. co-elution of isomers). Ion Mobility Separation, coupled with mass spectrometry, provides researchers an additional analytical dimension for the identification of such co-eluting isomers. For very dense mixtures, conventional ion mobility devices often do not have enough resolving power. The Trapped Ion Mobility Separation of the Bruker timsTOFTM offers high-resolution ion mobility separation together with a unique mobility expansion technology (imeXTM Technology).

This poster describes the application of HR trapped ion mobility-MS to the analysis of isomeric flavonoid mixtures as well as spiked and non-spiked propolis extracts. 

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