Detecting reactive oxygen and nitrogen species using EPR – from data acquisition to quantitative analysis
Some of the most abundant radicals produced in natural biochemical reactions are reactive oxygen species (ROS) such as hydroxyl, hydroperoxyl and superoxide radicals, and reactive nitrogen species (RNS), such as nitrogen monoxide and peroxynitrite.
In this webinar, Kalina Ranguelova, Applications Scientist for Bruker BioSpin, will outline how Bruker electron paramagnetic resonance (EPR) technology can be applied to the detection of ROS and RNS, focusing in particular on the use of spin trapping techniques.v
The webinar will highlight how Bruker’s EMXnano spectrometer, with its compact desktop footprint and easy-to-use functionality, makes this technology accessible to more labs and researchers than ever before.
What you will learn
This webinar will provide you with a background to how EPR works and spin-trapping techniques for analysing reactive oxygen and nitrogen species.
Key topics include:
- What is EPR?
- Reactive oxygen and nitrogen species
- Spin-trapping and probe techniques
- The Bruker EMXnano
Kalina Ranguelova was previously a researcher at the National Institute of Environmental Health Sciences where she studied EPR spin trapping and, since 2011, she has been an EPR Applications Scientist at Bruker BioSpin where she currently focuses on the analysis of beer, oil, wine, and pet food oxidation due to free radical processes.
During this webinar, she will outline how Bruker systems, including EMXnano can been applied to the detection and quantification of ROS and RNS.
The webinar will begin with an explanation of what EPR is and how it works and will touch upon its advantages over other techniques for detecting reactive species, such as chemiluminescent and fluorescent probes or assay-based methods.
Dr Ranguelova will then go on to discuss the methods of spin trapping and spin probes, approaches which help to overcome the short half-life of free radicals at room temperature and allow data on these reactions to be captured.
And by focusing on the EMXnano, Bruker’s most user-friendly desktop EPR instrument, Dr Ranguelova will highlight that EPR is a method that can be used by virtually anyone, with very little knowledge of EPR required.
Who should attend?
This webinar will interest anyone who wants to know more about the use of EPR for detecting ROS and RNS, including those who are coming to EPR for the first time. It is likely to be of particular interest for biologists, chemists, biophysicists and those working in academic research settings and pharmaceutical research and development.