Luxendo Light-Sheet Microscopes

TruLive3D Imager

The dual-sided illumination light-sheet microscope
TruLive3D Imager (Dual-sided Illumination Light-Sheet Microscope)

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TruLive3D Imager

Optimized for fast 3D multi-sample volume imaging of delicate live specimen in their native 3D environment, the TruLive3D Imager is especially suited for multi-position imaging of small embryos (e.g. mouse), 3D spheroids, organoids, oocytes and more, enabling time-lapse, high-throughput imaging experiments. The optical concept, with dual-sided illumination and single-lens detection from below, enables fast acquisition speed, high-resolution imaging and minimal shadowing effects.

Deep and Crisp Imaging
Dual-sided illumination and single-lens detection from below for fast acquisition speed, high-resolution imaging, and minimal shadowing effects.
Native and Natural
Close-to-natural environmental conditions for live-cell, long-term imaging of sensitive samples with reduced phototoxic effects.
Multiplexing
A large sample holder enables analyzing up to 100s of samples in one experiment with up to six different experimental conditions.

Caractéristiques

Compact, Robust and Vibration-Free Design

The TruLive3D Imager's compact, robust and vibration-free design provides maximal stability even during long-term and high-throughput experiments.

Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the quality of your sample is affected thanks to the unique and very gentle image-acquisition concept.

Deep and Crisp Imaging

Illumination and Detection

The TruLive3D Imager can achieve a resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.

The system features dual-sided illumination and single-lens detection from below, enabling fast acquisition speed, high-resolution imaging, and minimal shadowing effects. A wide-field imaging option facilitates sample positioning.

Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses project the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs.

Native and Natural

The TruLive3D Imager is equipped with environmental control. Temperature can be adjusted between 20–37°C for optimal incubation conditions.

In addition, the TruLive3D Imager also provides precise and stable environmental control (i.e. CO2, O2, N2, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO2, 1–21 % for O2, and 20–99 % for H2O (humidity). The gas humidifier offers feedback control for precise regulation.

The TruLive3D Imager is optimized for fast 3D multi-sample imaging of delicate live specimen in their native 3D environment.

Multiplexing

Large Sample Holder

The extended sample chamber in the TruLive3D Imager fits a large sample holder (length=75 mm) which can accommodate tens to hundreds of samples. It is designed for multi-position imaging of small embryos (e.g. zebrafish, Drosophila or mouse), 3D spheroids, oocytes and more.

The FEP foil lining the holder serves dual functions, a “curved coverglass” and a physical barrier between the immersion medium and sample medium.

TruLive3D Dish

The new TruLive3D Dish series of disposable sample holders seamlessly integrate into the system and allow testing different media conditions in one experiment.

The new ready-to-use TruLive3D Dishes are fully compatible with the TruLive3D Imager. The possibility to line up three disposable dishes extends the capacities of the system to enable the parallel imaging of samples grown under different experimental conditions.

Lux DATA - A Comprehensive Data Processing and Storage Solution

Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.

Applications

Live Sample Applications

Browse a selection of applications data from our customers below. Researchers are using the TruLive3D Imager in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.

Pancreatic Sphere

hESC-derived pancreatic spheres. Imaging on the TruLive3D Imager enables collecting information of several samples in one experiment. Visualization: Imaris (Bitplane)

Courtesy of:
Yung Hae Kim
Grapin-Botton Group, MPI-CBG
Dresden, Germany

 

Spécifications

Specifications

Illumination Detection
Effective Magnification
Field of View Pixel Size Optical Resolution
10x / 0.3 NA Nikon 25x / 1.1 NA

31.3x

62.5x

420 µm

210 µm

208 nm

104 nm

255 nm

Illumination Optics

  • Chromatic correction from 440 to 660 nm
  • Light-sheet generation by beam scanning
  • Flexible light-sheet thickness (2 µm to 6 µm)
  • Motorised ND filter set (90%, 10%, 1%) for high flexibility in using low laser power
  • Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens

Detection Optics

  • One Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
  • Two spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions) Filters adapted to the selected laser lines
  • Three motorised dichroic mirror positions for simultaneous dual channel acquisition
  • Motorised collar ring controlTwo high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
  • Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
  • Peak quantum efficiency (QE): 82% @ 560 nm

Accessoires

Accesories

TruLive3D Dishes

TrueLive3D Imager Sample Holder

Logiciels

Software

Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.

Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.

Electronics, Microscope Software and Computer

  • Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
  • Flexible GUI for interactive microscope control and experiment design
  • Computer with 256 GB RAM, Intel dual 8 core CPU
  • High-speed RAID controller for data streaming, 8×4 TB local storage in RAID 0
  • GeForce RTX 3070 graphics card
  • 10 Gbit/s on-board Ethernet port
  • 1 UHTV 43 inch display

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