Optimized for fast 3D multi-sample volume imaging of delicate live specimen in their native 3D environment, the TruLive3D Imager is especially suited for multi-position imaging of small embryos (e.g. mouse), 3D spheroids, organoids, oocytes and more, enabling time-lapse, high-throughput imaging experiments. The optical concept, with dual-sided illumination and single-lens detection from below, enables fast acquisition speed, high-resolution imaging and minimal shadowing effects.
The TruLive3D Imager's compact, robust and vibration-free design provides maximal stability even during long-term and high-throughput experiments.
Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the quality of your sample is affected thanks to the unique and very gentle image-acquisition concept.
The TruLive3D Imager can achieve a resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.
The system features dual-sided illumination and single-lens detection from below, enabling fast acquisition speed, high-resolution imaging, and minimal shadowing effects. A wide-field imaging option facilitates sample positioning.
Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses project the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs.
The TruLive3D Imager is equipped with environmental control. Temperature can be adjusted between 20–37°C for optimal incubation conditions.
In addition, the TruLive3D Imager also provides precise and stable environmental control (i.e. CO2, O2, N2, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO2, 1–21 % for O2, and 20–99 % for H2O (humidity). The gas humidifier offers feedback control for precise regulation.
The TruLive3D Imager is optimized for fast 3D multi-sample imaging of delicate live specimen in their native 3D environment.
The extended sample chamber in the TruLive3D Imager fits a large sample holder (length=75 mm) which can accommodate tens to hundreds of samples. It is designed for multi-position imaging of small embryos (e.g. zebrafish, Drosophila or mouse), 3D spheroids, oocytes and more.
The FEP foil lining the holder serves dual functions, a “curved coverglass” and a physical barrier between the immersion medium and sample medium.
The new TruLive3D Dish series of disposable sample holders seamlessly integrate into the system and allow testing different media conditions in one experiment.
The new ready-to-use TruLive3D Dishes are fully compatible with the TruLive3D Imager. The possibility to line up three disposable dishes extends the capacities of the system to enable the parallel imaging of samples grown under different experimental conditions.
Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
Browse a selection of applications data from our customers below. Researchers are using the TruLive3D Imager in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.
hESC-derived pancreatic spheres. Imaging on the TruLive3D Imager enables collecting information of several samples in one experiment. Visualization: Imaris (Bitplane)
Yung Hae Kim
Grapin-Botton Group, MPI-CBG
||Field of View||Pixel Size||Optical Resolution|
|10x / 0.3 NA||Nikon 25x / 1.1 NA||
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.