Ultima In vitro banner image

Ultima In Vitro Multiphoton Microscope System

Leading advances in neuroscience with fast, deep multiphoton imaging.

The Ultima In Vitro Multiphoton Microscope System is the platform on which leading scientists have conducted breakthrough experiments in brain-slice studies related to neural signaling and neural network mapping. Ultima In Vitro provides simultaneous imaging and electrophysiology, plus uncaging and photostimulation for the complete stimulus and recording environment required for research by today’s neuroscientist.

 

Contact Us Download Brochure 

Ultima in vitro tool

Ultima In Vitro Applications

Ultima in vitro high res multifoeld imaging

High-Resolution Multifield Imaging


The Atlas Imaging module in Prairie View software simplifies setup of 2-D and 3-D automated montages. X,Y boundaries are easily setup through a thumbnail display. Tiles not containing areas of interest can be turned off, and the Z range for 3-D montages can be variable, allowing more efficient collection of data.

5x5 montage at 40x (covering area of 1175 um x 1175 um) of mouse kidney section with Alexa Fluor® 488 WGA, Alexa Fluor® 568 Phalloidin.


Pharmaceutical Study with Tissue Explants


Tissue explants provide a higher throughput model for studying tissue response to pharmaceutical agents. The FLIM option allows measurement of metabolic status through measurement of fluorescent lifetimes.





AJ Walsh, RS Cook, ME Sanders, L Aurisicchio, G Ciliberto, CL Arteaga, and MC Skala. Quantitative optical imaging of primary tumor organoid metabolism predicts drug response in breast cancer. Cancer Research (2014). Sep 15;74(18):5184-5194

Utlima In Vitro tissue explants
Ultima in vitro neuronal signaling

Neuronal Signaling in Brain Slices


Ultima In Vitro is the system of choice for studying neuronal signaling at the both the dendritic and neural network levels. When equipped with optional photostimulation galvanometers, Ultima In Vitro provides a complete optical workstation, allowing simultaneous imaging, photostimulation and electrophysiology recording.



EPSP recorded in the dendritic trunk in response to a single, high-power ungaging event at s1 (black) or s2 (grey) in brain slice; Mark T. Harnett et al., Nature 491, 599-602.


Volumetric Imaging of Cell Preparations


Ultima In Vitro provides high resolution and efficient volumetric imaging of cell cultures and cells in artificial matrices.


EPSP recorded in the dendritic trunk in response to a single, high-power ungaging event at s1 (black) or s2 (grey) in brain slice; Mark T. Harnett et al., Nature 491, 599-602.

Ultima in vitro volumetric imaging

Multi-Photon Microscopy for In Vitro Preparations

Flexible architecture to meet application requirements and future upgrades

Ultima In Vitro is the ultimate “slice rig”, having provided the platform on which leading scientists have conducted breakthrough experiments in brain slices related to neural signaling and neural networks for more than ten years.

Simultaneous imaging, electrophysiology and uncaging/photostimulation provides a complete stimulus/recording environment for today's neuroscientist. A system that works out of the box, and yet provides the flexibility for the most demanding protocols means Ultima will be your work horse system for years.

Features

  • Secondary scan path enables simultaneous imaging and photostimulation
  • Visible, UV, and IR inputs for photostimulation
  • Sub-micron positioning control for point photoactivation
  • Sub-millisecond spirals for somal actiavtion
  • Synchronization and triggering to enable simultaneous imaging, photostimulation, and electrophysiology
  • Integrated electrophysiology control and recording with up to 16 analog outputs/inputs

Biological Models

Ultima In Vitro is compatible with many different biological models, including:

  • Brain slices
  • Excised tissue
  • Cell cultures
  • Invertebrates

In Vitro Applications

Ultima In Vitro is well suited to a variety of scientific applications including:

  • Neuronal signaling
  • Tissue structural changes
  • Protein transport
  • Intracellular ion dynamics

Prairie View Software

Bruker's proprietary imaging software provides turnkey operation for imaging as well as synchronizing the Ultima system with external stimulus and experimental control devices.

Options

We offer several options for the InVitro Multiphoton Microscopes.