fungiplex candida

Fungiplex® Candida IVD PCR: When Time Matters

Invasive Candidiasis

Invasive candidiasis (IC) is difficult to diagnose and is associated with high morbidity and mortality. Blood cultures exhibit a sensitivity of only 50 % for diagnosing IC, and become positive late in the disease. Fungiplex Candida enables the rapid identification of Candida species from blood samples, without the need to wait for blood culture.

Clinical problem

Invasive candidiasis is a disease which affects immuno-compromised and critical care patients. Conventional diagnostic techniques are not compatible with the timely diagnosis of IC, and exhibit poor sensitivity. Delays in initiating antifungal therapy in patients with candidaemia dramatically increase mortality rates. Empiric treatment is often used.

  • Blood cultures exhibit poor sensitivity for diagnosing invasive candidiasis, and become positive late in the disease
  • Delayed diagnosis of IC is associated with increased mortality
  • Empiric treatment is often employed, which results in overuse of antifungal agents

PCR has shown promise for Candida detection, with improved diagnostic accuracy compared to culture and other biomarker tests, providing results within hours.

Molecular testing for Candida

Fungiplex Candida is a multiplex real-time PCR assay, which rapidly detects the main causative species associated with IC, and differentiates those resistant to first-line treatment:

  • Candida spp. (C. albicans, C. parapsilosis, C. dubliniensis, C. tropicalis)
  • Candida glabrata
  • Candida krusei

Direct testing

Fungiplex Candida is validated for use on DNA extracted from whole blood, serum and plasma without the need for culture.

Rapid results

Fungiplex Candida provides results in under 2 hours from extracted DNA, allowing rapid reporting from the laboratory to support clinical decision-making when time matters.

Compatibility

Fungiplex Candida is provided in an easy to use master-mix format and is designed to be compatible with existing laboratory equipment, minimising the need for dedicated bench space or additional training:

  • ABI 7500
  • ABI QuantStudio 5
  • Bio-Rad CFX
  • Qiagen RotorGene
  • Roche Lightcycler 480 II (with colour compensation kits)

 

Please contact us to discuss validating Fungiplex Candida on your thermocycler if it is not listed.

Fungiplex Candida is designed to run on the same thermocycler conditions as Fungiplex Aspergillus, allowing clinics and laboratories to conduct testing with flexibility and efficiency.