PepSep® Content Collection

Overview

PepSep® Columns and Emitters are designed to deliver high-quality, fast, and reproducible separation of peptides and proteins using reversed-phase HPLC. These columns are essential for proteomics applications, including single-cell proteomics, immunopeptidomics, and high-throughput proteomics. They are known for their robustness, speed, and sensitivity, making them a preferred choice in academic and core labs.

The robust design of these columns ensures reliable performance, even with challenging samples. Available in a variety of sizes and configurations, PepSep® Columns can meet the specific needs of different proteomics applications.

Materials

Key Features

  • High Throughput: Designed for high-throughput applications, allowing for faster analysis and increased sample throughput.
  • Superior Sensitivity and Resolution: Provides high sensitivity and resolution, essential for detailed proteomics analysis.
  • Minimal Backpressure: Enables higher nanoflow rates with minimal back pressure, translating to quicker analysis times.
  • Reproducibility: Ensures consistent and reproducible results, crucial for reliable data collection.
  • Ease of Use: Features a straightforward twist-on and twist-off mechanism, this column design ensures effortless connection to all CaptiveSpray sources utilizing CaptiveSpray 2 Emitters. The two-part solution, with a separate emitter, guarantees compatibility with all LC-MS systems.
  • Versatility: Suitable for a wide range of proteomics applications, from routine to complex sample types.

PepSep® Portfolio

  • PepSep® Advanced: Designed for consistent, high-quality protein and peptide separation.
  • PepSep® Classics: Reliable and versatile columns designed for routine proteomic analyses.
  • CaptiveSpray 2 Emitters: Perfect addition to PepSep® columns when using a CaptiveSpray source, offering ready-to-use fused silica emitters with zero dead volume.
  • CSI Transfer Line Kit: Simple and leak-tight connection of syringes to all CaptiveSpray Sources for direct infusion, calibration and troubleshooting.

"Impressed by the chromatography delivered from the very first run. Sharp, sensitive and reproducible performance at a reasonable price. Don’t  have to think about them, they just work and keep on working."

Lynn Spruce, Technical Director, CHOP-PENN Proteomic Core Facility, Philadelphia, PA, USA

 

For Research Use Only. Not for use in clinical diagnostic procedures.