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B.I.QUANT-PS 2.0™ Bruker IVDr Quantification in Plasma/Serum

The Bruker IVDr platform is optimized towards supporting the analysis of epidemiological studies with large scale, dedicated clinical and translational trials, and high throughput Biobank quality control. Based on nuclear magnetic resonance (NMR)spectroscopy, the standardized Bruker IVDr Methods module(B.I.Methods) guarantees high reproducibility and transferability under full automation at anytime and anywhere what is not achievable by any other analytical tool.

This standardized platform allows to combine different solutions.For plasma/serum, it means Bruker IVDr Lipoprotein Subclass Analysis (B.I.LISA™) and automated quantification of small molecule metabolites (B.I.QUANT-PS™) using the same experiment. Now we upgrade the quantification from26 metabolites up to 41 metabolites in B.I.QUANT-PS 2.0.

Requirements for B.I.QUANT-PS 2.0

  • ƒƒBruker IVDr platform or compatible AVANCE™ III HD or AVANCE NEO 600 MHz systems
  • ƒƒUse of B.I.Methods module and SOPs for plasma/serum for sample preparation and measurementƒƒ
  • Regular quality control of the absolute temperature, solvent suppression and quantification reference sample (preferably daily using automated quality control tool including in B.I.Methods)
  • ƒƒAccess to Bruker Data Analysis server for fully automated remote analysis (transfer of spectra after measurement to Bruker server via private ftp, back-transfer of result PDF report and XML file)

Benefits of B.I.QUANT-PS 2.0

  • Ease of use, simple and rapid sample preparationƒƒ
  • Fully automated quantification of 41 metabolitesƒƒ
  • Different analytes classes can be quantified simultaneously in a single runƒƒ
  • Absolute concentration for each metabolite is given due to the calibration with one Quantification Reference sample(B.I.Methods™)ƒƒ
  • Validation of all LODs has been done following ISO 17025 guidelines for wet spiking
  • ƒƒRaw concentration and additionalƒƒ quantification result assessment information (correlation and residue) for each metabolite is given even below LOD
  • ƒƒInteractive PDF report allowing visual fit ambiguity assessmentƒƒ
  • Rapid analysis: daily 120 samples can be prepared, measured and analyzedƒƒ
  • Retrospective analysis possible if the sample was prepared and measured using B.I.Methods moduleƒƒ
  • Works on plasma or serum sample

Further Information

Deliverables B.I.QUANT-PS 2.0

  • ƒƒIncludes 41 metabolites (free metabolites, no protein denaturing done) with concentration ranges, occurring inmost human plasma/serum samples
  • ƒƒIncludes matrix identity test, differentiation between EDTA plasma and heparin plasma or serumƒƒ
  • In the analysis report, the metabolites are grouped according to their chemical class

    • Alcohols and derivatives
    • Amines and derivatives
    • Amino acids and derivatives
    • Carboxylic acids
    • Essential nutrient
    • Keto acids and derivatives
    • Sugars and derivatives
    • Sulfones
    • Technical additives

Figure 1

In table 1 an extract of the report automatically generatedby B.I.QUANT-PS 2.0 is shown. Metabolites are sorted intochemical classes. Absolute quantification values are given,LODs are listed as well as a 95% concentration range derivedfrom the validation spectra set. The actual sample is shownas a black bar in the concentration range, if the correspondingmetabolite is detected.

Table 1

Extract of B.I.QUANT-PS 2.0 of a serum

Figure 1 shows the interactive graphics of Alanine on aB.I.QUANT-PS 2.0 PDF report. It is an ideal situation, wherethe fit corresponds fully to the metabolite signal well aboveLOD, the raw concentration (r) is close to the result concentrationand the correlation (ρ) is > 95% and the residue (Δ) isclose to zero mmol/L.

Figure 2

Validation of Plasma/Serum Quantification Method

To test the validity of the results, the following measureswere taken and continuously followed:„„

  • Test reproducibility of measurement and analysis
  • „„Test transferability of measurement and analysis(participation of multi-laboratory trial)
  • „„Recovery rate (wet spiking)„„
  • Comparison to conventional methods
  • „„Traceability tests on some metabolites

For detailed metabolite lists and more technical information,please contact manfred.spraul@bruker.com and for sales information your local sales representative.B.I.QUANT-PS 2.0 module is offered as flat rate for 1 year,3 years with remote result generation and as pay per use transferring data sets to be analyzed by email or other means.

Figure 2 shows the interactive graphics of phenylalanine,where the raw concentration is below LOD. Using the graphical figure displayed, the fit signal can be clearly discriminated from the rest of the spectrum and the correlation is > 95%. The raw concentration can be used as approximative concentration estimate.

The results are provided twice, in PDF format as well as in XML format, which allows easier transfer into other programs for statistical analysis.


The B.I.QUANT-PS 2.0 module is for research use only and is
not released for clinical diagnostics.

Download IVDr Anaysis Reports

To download sample reports please complete a short form here.