Triple Resonance CryoProbe for Protein Applications

Proton-optimized triple resonance NMR ‘inverse’ probe

The TCI CryoProbe is a proton-optimized triple resonance NMR ‘inverse’ probe, featuring three fully independent channels (plus lock channel) for simultaneous decoupling on multiple nuclei such as 13C and 15N.

Triple Resonance Probes give you access to advanced NMR techniques, letting you pulse up to four nuclei in one experiment. The TCI CryoProbe is optimized for 1H and 13C observation. If you add a deuterium lock switch, you can decouple 2H, as well, permitting decoupling of up to four nuclei in a single pulse sequence. Triple (TCI) Resonance Probes are used extensively for the family of double and triple resonance experiments developed to perform NMR structure determination of biological macromolecules.

TCI CryoProbe is equipped with cold preamplifiers for 1H and 13C. Its design in conjunction with the cryogenically cooled preamplifier for deuterium channel guarantees highest sensitivity for the lock channel, resulting in excellent stability of the spectrometer. This also permits the use of as little as a few percent of deuterated solvents. The probe is compatible with shaped tubes for optimum performance on lossy samples.

3 is the new 5

This new design of smaller diameter NMR CryoProbes is advantageous for highest-field GHz biomolecular applications, when sample volumes are limited and sample salt concentrations are in the physiological range. The new 3mm design also delivers shorter radiofrequency (RF) pulses at equivalent RF power, compared to 5 mm CryoProbes, an important benefit for IDP dynamics experiments, when working at GHz fields. 

Professor Göran Karlsson, Director of the Swedish NMR Center at the University of Gothenburg, was Bruker’s key collaborator in the development of 3mm CryoProbes. He commented: “The performance of the new 3 mm TCI CryoProbe on our 800 MHz is just spectacular. For some time we have been working with 3mm samples in structural biology, and with the new 800 MHz 3mm CryoProbe we obtain dramatic S/N increase. In metabolomics applications, we benefit from both, the increase in S/N and the ability to work with reduced sample volumes. For many applications in life science research, the 3mm CryoProbe represents a leap forward. We’re planning to order the 3mm CryoProbe also for our 900 MHz spectrometer."