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Standard Bore Imaging Probe

Micro 5

The micro-imaging probe Micro 5 has been developed for applications with standard bore magnets (52 mm ID) for the investigation of small objects with diameters of < 1 mm to max. 10 mm.

Applications

The Micro 5 probe is typically used to study mineral or geologic samples, polymers, tablet dissolution kinetics, plants and seeds, bone or tissue biopsy samples, and small insects.

Gradient System

Bruker’s Streamline gradient technology provides excellent active shielding and high efficiency for X, Y, Z gradients of 300 G/cm at 60 A current. The very low resistance (< 0.2 Ohms) minimizes internal heat dissipation, and with water cooling very high duty cycles can be employed with switching times down to 50 µs. A built-in temperature sensor and security circuits guarantee for safe and reliable operation. The internal diameter of the gradient system is 19 mm, and excellent linearity is achieved over a large volume (15-mm diam. sphere) so that quantitative image analyses as well as precise diffusion experiments can be performed.

The probe features a modular construction with exchangeable rf-inserts of various designs and variable temperature operation and control. The Micro 5 has a removable gradient system and uses the same body and electronics as the Diff 30 probe.

RF Section

The probe body is equipped with two independent rf-channels. The exchangeable rf-inserts are mounted on ceramic bases and are offered as single- or double-tuned units in two basic forms (horizontal solenoid or vertical Helmholtz) with various diameters.

In addition Coil-on-a-Chip inserts are available for this probe.

DIFF30 / DIFF50 Configurations

The Micro5 standard bore micro-imaging probe can be converted into a Diff30 standard bore z-diffusion probe or into a Diff50 wide bore z-diffusion probe, just by exchanging the plug in Micro5 gradient system ba a Diff30 or Diff50 gradient system. With the Diff30 configuration gradient strengths of up to 1800 G/cm are available. With the Diff50 configuration gradient strengths of up to 3000 G/cm are available.

Micro 5 Probe and Gradient Features

Gradients:xyz
Gradient Strength:4.8 G/cm/A
ID/OD:19/40 mm
Linearity:
+/- 1.3% peak-peak
18 mm sphere
+/- 1.6% peak-peak
19 mm sphere
+/- 2.1% peak-peak
20 mm sphere
Inductance:10 - 20 µH
Resistance:<= 120 m?
Rise Time, 0-60A, 120V:< 50 µs
Cooling:water
Maximum Current Tested:60 A
Rf Coil Types (exchangeable):solenoid, saddle, microcoils
Rf Coil Diameters:up to 10 mm
Nucleus:1H and/or X

Saccharose Transport in Anigozanthos Plants

13C Inverse detected Saccharose Transport in Anigozanthos Plants.

Detection of sugar transport in the Anigozanthos stem to determine the nectar production mechanisms. The plant was fed with 13C labelled glucose.

The C1-a bound protons (coloured) overlayed to the proton image. The absorbed sugar can be found in the vascular bundles only and is not stored in the tissue. 400 MHz, 9.4 T, Cryo probe for micro-imaging.

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Courtesy of M. Wenzler and B. Schneider, Max Planck Institut für chemische Ökologie, Jena, Germany

Cooking of Spaghetti

Spaghetti were cooked outside of the magnet with four different cooking times and then mounted together with a uncooked one for comparison in the imaging probe.

Cooking times: (1) 0 min, (2) 1 min, (3) 3 min, (4) 5 min, (5) 10 min

The cooking process of pasta is visualized by three easy distinguishable zones in cross section images through spaghetti, indicating volumes of uncooked, intermediate and finished cooking.

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Courtesy of C. Zweifel and C. Handschin, ETH Zürich

Dissolution of Tablets

The dissolution and the drug release of tablets is controlled by various properties: tablet material, morphological tablet structure, surface material, properties of the gel layer during dissolution, ph value of the environment during dissolution etc.

The dissolution process can be visualized by time resolved 2D and 3D images, observing the water protons.

The image artefact at the left side of the dark tablet shape is caused by a paramagnetic colour  from the letters of the trademark, written on the tablet surface.

The dissolving process starts at one position, where the outer cover of the tablet was dissolved first. After the start a faster dissolving process continues.

dissolution.png
Courtesy of Peter Hummel