With up to 80,000 resolution, sub ppm mass accuracy, True Isotopic Patterns & industry leading sensitivity at the subunit level, Bruker UHR-QTOFs provide confident characterization first time, every time. Isotopic resolution of antibody subunits allows clear interpretation of protein modifications and routinely delivers monoisotopic masses at better than 2ppm mass accuracy. Bruker ESI-QTOFs offer a middle-up solution enabling UHPLC speed analysis with no sensitivity or resolution compromises, uniquely allowing simultaneous characterization of product & impurities in a single analysis.
ESI-QTOF middle-up analysis is easily complemented by adding sequence information via middle-down experiments, which also benefits from the same minimal sample preparation. The maXis II ETD can provide confirmation of N- and C-terminal sequences, whilst LC-MALDI can be used for validation of the full subunit sequences. These approaches can be supplemented with intact mass and middle-up measurements to provide conclusive information for sequence variant detection and biosimilar development.