Rabbit Lymph Node Tissue

MALDI Imaging - Direct imaging of proteins, peptides, lipids, drugs and metabolites in tissue

MALDI Imaging allows direct measurement of mass spectra from tissue. It can directly measure proteins, peptides, lipids, drugs, and metabolites. The distribution of the detected compounds can be visualized as an image.

See a video for a comprehensive overview of the workflow.

 

Molecular histology

The histological context of a molecular measurement is crucial for the correct interpretation of molecular imaging data. Conventional histological staining can be performed on the same tissue section. Bruker’s unique software can overlay and seamless cross-fade a high resolution virtual microscopy slide with the molecular image. This effectively turns MALDI Imaging into standard histology with an added molecular dimension.

For more information, see the video demonstrations of a rat testis and a pancreas dataset.

 

Targeted and untargeted molecular imaging and biomarker discovery

MALDI Imaging can be used for both targeted and untargeted analysis. A typical example of a targeted analysis is the measurement of drug and metabolite distribution in tissue. A typical example of an untargeted analysis is the search for protein or lipid biomarkers.

 

Drug and metabolite imaging

MALDI Imaging allows the measurement of drug distribution in tissue. Furthermore it allows the molecular differentiation of the parent drug and metabolites. The combination of MALDI Imaging with the histology gives additional insight and allows to bridge biology and chemistry in drug development.

 

Clinical biomarker discovery

Cancer samples often are highly heterogenous with a lot of different histological structures on a rather small scale. Since MALDI Imaging preserves the spatial distribution and the histology of the samples, it is perfectly suited for the analysis of human cancer samples. MALDI Imaging has been successfully applied to the detection of previously unknown proteins in cancer. Moreover, molecular signals or patterns have been shown to correlate with tumor subtypes or other parameters such as outcome or susceptibility to treatment.

 

Other application areas of MALDI Imaging

Other application areas of MALDI Imaging include model studies in neuroscience, developmental biology, molecular interactions of bacterial colonies, distribution of secondary metabolites in plants and others.

 

 

"For research use only. Not for use in diagnostic procedures!"