MALDI Imaging allows direct measurement of mass spectra from tissue. It can directly measure proteins, peptides, lipids, drugs, and metabolites. The distribution of the detected compounds can be visualized as an image.
See a video for a comprehensive overview of the workflow.
The histological context of a molecular measurement is crucial for the correct interpretation of molecular imaging data. Conventional histological staining can be performed on the same tissue section. Bruker’s unique software can overlay and seamless cross-fade a high resolution virtual microscopy slide with the molecular image. This effectively turns MALDI Imaging into standard histology with an added molecular dimension.
For more information, see the video demonstrations of a rat testis and a pancreas dataset.
Targeted and untargeted molecular imaging and biomarker discovery
MALDI Imaging can be used for both targeted and untargeted analysis. A typical example of a targeted analysis is the measurement of drug and metabolite distribution in tissue. A typical example of an untargeted analysis is the search for protein or lipid biomarkers.
Drug and metabolite imaging
MALDI Imaging allows the measurement of drug distribution in tissue. Furthermore it allows the molecular differentiation of the parent drug and metabolites. The combination of MALDI Imaging with the histology gives additional insight and allows to bridge biology and chemistry in drug development.