The pattern of modifications on a protein can have significant influence on its functionality, activity level, solubility, or it’s half-life. The ability to accurately detect, profile and characterize post translational modifications (PTMs) on proteins in a sample is essential to the understanding of biological systems.
Comprehensive protein and PTMs characterization raise several challenges, including, maximizing sequence coverage, detecting low abundant modified peptides and dealing with the fragmentation challenges encountered by specific modifications like glycosylation or phosphorylation.
Maximizing sequence coverage is a must for applications ranging from protein product production control to the mapping of PTMs in a shotgun protoemics experiment. The combination of the additional ion mobility separation and of the timsTOF Pro’s high quality MS/MS spectra @ > 100 Hz is key to maximizing the number of fragmented peptides and hence the sequence coverage of a protein. For antibody or biosimilar drug production control, for instance, 100% sequence coverage of both the heavy and hight chain can be obtained from a very short 10 min gradient using the timsTOF Pro.
Maximizing sequence coverage is a must for applications ranging from protein product production control to the mapping of PTMs in a shotgun Proteomics experiment.