The Bruker microfluidics unit can be used with Vutara to address any localization microscopy application where a large number of targets needs to be imaged. Fifteen probe vials are provided, and the system allows probe vials to be interchanged during experiments, thus allowing an unlimited number of targets.
Example localization microscopy applications:
- DNA PAINT
- Multiplexed antibody labelling
- Refresh STORM
The Bruker microfluidics unit is also compatible with the Opterra confocal for serial labelling confocal applications.
Dimensions: 370mm x 300mm x 204mm
Reservoirs: 15 2.0 ml Eppendorf tubes for probes
Wash/buffer reservoirs: Two 15 ml, one 50 ml, one 100 ml
Flow rates: 1 µL/min – 1000 µL/min
Pumping method: Air pressure (self-contained pump)
Interchangeability: Reservoirs can be changed out while system is in use for an experiment; allows an unlimited number of probes to be used.
Dead volume: Fluidics unit: 85 µL for probe channels, 190 µL for wash/buffer channels
Tubing from fluidics unit to flow chamber: 2 µL/cm
Flow cell: 150 µL for standard flow cell