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Opterra Xenopus Cilia

Live-Cell Imaging: Enabling 4D Fluorescence Confocal Microscopy

The Opterra II swept-field confocal microscope utilizes proprietary one-dimensional pinhole array technology to combine the resolution of traditional confocal systems with the speed typically associated with wide-field imaging. 

 

With its short acquisition times and cell-protecting minimization of photobleaching and phototoxicity, Opterra II is ideal for advanced live-cell imaging.


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Opterra II Live-Cell Imaging


Benefits That Spinning Disk Confocal Simply Cannot Match


Keep highly sensitive specimens alive, and maintain their cellular function very close to natural biological conditions. Opterra II is ideal for studies ranging from protein localization and trafficking to nuclear, microtubule, and vesicle dynamics.


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Z-stack comparison of signal-to-noise ratio through a 200 um gel embedded with collagen fibers.  Left: spinning disk system. Right: Opterra II.  Sample courtesy of Brian Burkel, University of Wisconsin.

10 frames per second imaging of Xenopus GRP cilia. GFP labeled cilia, RFP membrane marker. Courtesy of Melanie Tingler, Shiaulou Yuan, and Mustafa Khokha. Cold Spring Harbor Xenopus Course, Yale University


Flexibility in Speed, Resolution, and Fluorescence Intensity



Optimize experimental conditions across an array of life science applications. Opterra II utilizes a unique scanner design that contains both pinholes and slits, which enables flexible adjustment between speed, resolution, and intensity.



Field Uniformity for Quantitative Analysis of Acquired Images in All Dimensions



Perform quantiative measurements of fluorescent intensities across and between images. Opterra II's guaranteed field uniformity works with multiple dichroics and provides unmatched quantitative performance right out of the box.

Opterra Imaging Stage Montage
Montage of 2 mm x 1.5 mm area at 60x, pancreas tumor section.

Zebrafish embryo, 8 hour timelapse, 80 plane stacks every 5 minutes


Low Phototoxicity/Bleaching for Time-Lapsed Volumetric Studies



Control the dose of excitation light by minimizing exposure time and delivering light to specific locations only when needed. Opterra II enables research on highly sensitive samples, keeping specimens alive and maintaining cellular functions close to biological conditions.


Ultrafast Imaging at the Speed of Life



Capture rapid physiological processes in real-time without missing fast transient events. The enhanced swept-field confocal scanning technology of the standard Opterra II allows for high-speed imaging at hundreds of frames per second. With an appropriate camera, over a thousand frames per second can be achieved

 125 frames per second imaging of Xenopus red blood cells in gills. Playback at 5x slow motion. Video courtesy of Vaughn Colleluori and Mustafa Khokha, Cold Spring Harbor Xenopus Course,Yale University.

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