The three dimensional organization of chromosomes plays a key role in chromosomal function. Chromosomes exhibit structural differences on a regional basis, both within and between chromosomes. Understanding these structural differences likely will prove useful in understanding chromosomal function in both normal and pathological states.
Genomics, OligoSTORM, and OligoDNA-PAINT
Based on the use of OligoSTORM (B. J. Beliveau et al., 2015), the laboratory of Ting Wu at Harvard University, in collaboration with Bruker, has developed a method for imaging and visualizing DNA sequences in specific chromosomal regions at the super resolution level using the Vutara 352 (view webinar). Specially designed oligonucleotides are hybridized to a chromosomal sequence. By sequentially labelling a sample with secondary oligos and then imaging with localization microscopy, a 3-D image of the structure of that labeled region can be generated.