B. fragilis and carbapenem resistance
Carbapenem resistance in B. fragilis is frequently associated with presence of the cfiA gene, encoding a metallo-beta-lactamase conferring resistance to nearly all beta-lactam antibiotics. As a result infections with B. fragilis cfiA positive strains are very difficult to treat. Different studies show the increase in the resistance to carbapenems of B. fragilis and also the increased frequency to harbour the cfiA gene silently.
Automated detection of cfiA positive strains
Prerequisite for initiation of the cfiA detection process is successful identification of B. fragilis in the MBT identification workflow. The MBT Subtyping Module looks then automatically for specific peaks associated with the protein expressed by B. fragilis cfiA positive strains. If present, the best match for the respective sample will be reported as a presumptive cfiA positive strain.
Not for use in clinical diagnostic procedures.