Often overlooked, but of great importance is the sample preparation. Not only the positioning of the sample in the microCT scanner, which will allow you to obtain the desired pixel size, but also the stability of the sample throughout the scan is crucial. Samples such as fibers or micro-spheres can be stably mounted on the provided metal sample holders by using a small drop of glue. After the glue is dry, this easy and straightforward technique will enable long scans without sample movement. If the samples are preserved in ethanol of formaldehyde, placing them directly on a stage will allow the liquid to evaporate during the scan, resulting in artefacts in the reconstructed images. Leaving the sample surrounded by liquid will increase the noise in the image and will impact the beam hardening. By wrapping the samples in parafilm or low dense wax, thereby creating a ‘cocoon’, will prevent this.