The aqueous extracts of QHYH were added to a DMPO/OH• adduct generating system at varying concentrations. A Bruker X-band EPR spectrometer was used to detect free radicals in treated mouse cerebral cells also treated with high amounts of glucose.
The Fenton reaction system was used to generate OH• free radicals. Aqueous extracts of QHYH and its individual ingredients were added to the Fenton reaction system, the mixture was then inserted into a flat quartz cell. Then, researchers measured EPR spectra following the addition of Fe2+/DTPA after 5 minutes.
In a concentration-dependent manner, a significant decrease was noted in the DMPO/OH• adduct signals. Results indicated that the scavenging ability of QHYH was around 82.2%, significantly higher than the individual components.
Overall, the use of Chinese medicine in reducing free radicals associated with diabetes shouldn’t be discounted. QHYH, in particular, shows promise in vitro for combating high levels of free radicals associated with hyperglycemia, which may play a role in the prevention of endothelial dysfunction by protecting endothelial cells from oxidative stress.