Interfacing Chromatography to NMR

Hyphenated analytical techniques combining mass spectrometry and chromatography are well established laboratory tools. The combination of chromatography and NMR has also made its way into the analytical laboratory. Further developments even combine all three techniques into an LC-NMR/NMR-MS system and the introduction of solid phase extraction as efficient interface between chromatography and NMR. In principle, two different main workflows are possible.

L C Schema

In the currently supported workflow the chromatography system is coupled directly to the NMR spectrometer. The sample is transferred into the NMR "as is", that is, it arrives with the concentration and the solvent as supplied by the chromatography system. The NMR spectra are then acquired in on-flow mode (continuously, while the chromatography is running).

LC NMR system

Connection to the NMR Probes - Flow Probes and CryoFit

For the NMR measurements of a directly coupled LC-NMR/MS systems a flow probe is required. Due to the required high sensitivity and the typically low sample concentrations elution from a chromatography system typically CryoProbes™ are used. They provide a dramatic increase in sensitivity by a factor of 3-4 in tube operation mode. Bruker offers a CryoFit™, which converts any standard 5-mm CryoProbe or a CryoProbe Prodigy into a flow probe. The conversion takes only a few minutes and is done while CryoProbes is in the magnet and cooled. No downtime for warm up/cool down required.

For existing LC-SPE systems Bruker offers a solution to elute samples in to NMR tubes for measurement under standard conditions, i.e. without usage of flow equipment. This workflow is described on the LC-SPE-TT page.

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