For the NMR measurements of a directly coupled LC-NMR/MS systems a flow probe is required. Due to the required high sensitivity and the typically low sample concentrations elution from a chromatography system typically CryoProbes™ are used. They provide a dramatic increase in sensitivity by a factor of 3-4 in tube operation mode. Bruker offers a CryoFit™, which converts any standard 5-mm CryoProbe or a CryoProbe Prodigy into a flow probe. The conversion takes only a few minutes and is done while CryoProbes is in the magnet and cooled. No downtime for warm up/cool down required.
For existing LC-SPE systems Bruker offers a solution to elute samples in to NMR tubes for measurement under standard conditions, i.e. without usage of flow equipment. This workflow is described on the LC-SPE-TT page.