MRI クライオプローブ用途ギャラリー

小動物MRIのSNRに新しい展開

15.2 T mouse brain anatomy

Very high spatial resolution: In-plane (19.5 µm)², slice thickness: 150 µm, while maintaining good contrast and signal-to-noise ratio. Figure 5: Images located approximately at position Bregma -2.7 mm. (a) Full field of view FLASH image, (b) shows an expa

: Very high spatial resolution: In-plane (19.5 µm)², slice thickness: 150 µm, while maintaining good contrast and signal-to-noise ratio. Figure 5: Images located approximately at position Bregma -2.7 mm. (a) Full field of view FLASH image, (b) shows an expa

: (a,d) Expanded FLASH images, (b,e) phase images of the very same FLASH images, and (c,f) corresponding Nissl stained plates. Images (a-c) Bregma -2.7, (d-f) Bregma -1.9. MRI acquisition details: Resolution in-plane: (19.5 x 19.5) µm², field of view: (1.5

: (a,d) Expanded FLASH images, (b,e) phase images of the very same FLASH images, and (c,f) corresponding Nissl stained plates. Images (a-c) Bregma -2.7, (d-f) Bregma -1.9. MRI acquisition details: Resolution in-plane: (19.5 x 19.5) µm², field of view: (1.5

: Six full field of view coronal RARE slices of mouse brain in vivo. Acquisition details: RARE, 6 echoes, resolution: (29 x 29 x 200) µm³, TR: 3500 ms, TE: 25.3 ms, scan time: 25 min 40 s. Picture made at 15.2T field strength

: Direct comparison of high-resolution in vivo mouse brain images with Nissl stained plates. Resolution in-plane: (29 µm)², Slice thickness: 200 µm. (a) Full field of view coronal RARE image. Picture made at 15.2T field strength

: Direct comparison of high-resolution in vivo mouse brain images with Nissl stained plates. Resolution in-plane: (29 µm)², Slice thickness: 200 µm. Image (b) shows an expanded view of the hippocampal area, and image (c) a corresponding Nissl stained plate

: 3D Time of Flight (ToF) angiography in vivo without contrast agent, (a) MIP rotating around AP axis. Acquisition details: GEFC, TR: 37 ms, TE: 3.3 ms, matrix: 384 × 256 × 256, resolution: (49 µm)³ isotropic. Picture made at 15.2T field strength

: 3D Time of Flight (ToF) Angiography in vivo without contrast agent, (b) MIP rotating around LR axis. Acquisition details: GEFC, TR: 37 ms, TE: 3.3 ms, matrix: 384 × 256 × 256, resolution: (49 µm)³ isotropic. Picture made at 15.2T field strength

: In vivo murine brain micro-imaging with resolutions slowly approaching cellular levels becomes feasible at high magnetic field strengths (15.2 T) using a cryogenic RF coil. This may open up new possibilities for research on microstructural changes in gene

: The full cycle of a beating mouse heart in MIP representation. IntraGate self-gated angiography (work-in-progress)

: Maximum Intensity Projection of mouse spine with MRI CryoProbe. Visualization of posterior intercostal arteries and veins.

: Gray-white matter differentiation in fractional anisotropy map. Clear identification of nerve fibers (yellow arrows) in the color-coded diffusion tensor display.

: High resolution T2- weighted TurboRARE mouse spine images. Excellent differentiation of gray and white matter.

: Triggered Snapshot Time-of-Flight angiography. Visualization of liver vessels with Maximum Intensity Projection

: SWI processed FLASH image of a subcutaneous mouse tumor, and the corresponding RARE reference image.

: Direct detection of glucose metabolism in mouse brain. After overnight fasting, a bolus of 130 mg/kg [1-13C] glucose was given within 20 sec, f4.

: STEAM localized 1H spectroscopy in mouse frontal brain. Minimal evolution of VTJ-coupled resonances due to short echo time (3 ms); Optimal for investigation of macromolecules.

: Subcutaneous tumors in mice; Correlation of susceptibility weighted imaging and T2* maps. SWI indicated tumor vascularization. Precise partitioning of the tumor.

: Improved definition of small structures such as vessels and fibers compared to standard T2*-weighted protocols.

: AD plaque imaging in a 24 month old mouse in both T2- and T2*-weighted contrast. Exemplary plaques are marked with red arrows.

: Courtesy Aline Seuwen, ETH Zurich

: 3D Flash: 40*40*60 μm, TR=400, TE=7.6, α= 30°, 1h17min. Courtesy Aline Seuwen, ETH Zurich

: FOV: 1.6*1.6 cm, Mtx: 384*384, Res: 42*42 um, Slice th.: 150 um, TE: 5.5 ms, TR: 400 ms, Acqu. Time: 25 min

: FOV: 1.96*2.54 cm, Mtx: 512*512, Res: 38*50 um, Slice th.: 300 um, TE (eff): 46.3 ms, TR: 3500 ms, Acqu. Time: 1h

: Upper row: 2D Gradient echo, TE/TR= 5.5/400 ms, α = 30°, 40x40x160 µm, 21 min. Lower row: 2D spin echo,TEeff/TR= 45/3500 ms, 42x44x300 µm, 21 min, Courtesy Aline Seuwen, ETH Zurich

: Courtesy Aline Seuwen, ETH Zurich

: Voxel location: Pre-frontal Cortex, Acquisition: PRESS, Voxel volume: 3.2 µl, SNR Gain (Cryo/RT): ~ 1.7. Courtesy Aline Seuwen, ETH Zurich