Ultimate Force Control for Imaging of Dynamic Processes
In AFM studies of biomolecular self-assembly, individual molecules must be loosely bound to a substrate (eg. mica) to allow them to undergo the conformational changes required to form proper intermolecular bonds. However, this makes AFM imaging challenging, as these highly mobile molecules can easily be distorted or dislodged from the substrate surface.
Such is the case in DNA quadruplex formation. The highly sensitive force control of Fast Tapping allows one to follow the folding and unfolding dynamics of the quadruplex region at imaging speeds of 1 fps without distorting or dislodging the DNA. The ability to study the formation and resulting structures of these DNA quadruplexes can help to better understand their related biological functions.