The PeakForce QNM images shown here are of live MDCK cells obtained on the BioScope Resolve – with the topography image on the left and the corresponding modulus image on the right. The colorscale in the modulus image corresponds to increasing modulus values – with darker colors having lower modulus and lighter colors having higher modulus. Keeping this in mind, when looking at the modulus image we see long, thin structures corresponding to actin fibers appear to have higher modulus – being lighter in color - while cell surface features, which we believe to be microvilli, appear softer - or darker in color - than the cell membrane itself.
PeakForce QNM topography image (left) and corresponding modulus image (right) of living MDCK cells. Cell structures corresponding to actin fibers show higher modulus (lighter) while cell surface features, believed to be microvilli, appear softer (darker)