The dynamics of CM15 antimicrobial activity on the native outer membrane of live E. coli cells was observed by high speed AFM with nanometer resolution. The movie was recorded at 8 seconds/frame with each frame captured at a scan size of 300nm with a resolution of 1024x256 pixels. High-resolution imaging of the outer membrane surface of individual live E. coli cells revealed the presence of nanometer-sized membrane-associated features that exhibited an ordered, lattice-like structure. These structures are similar to those previously observed on excised patches of bacterial outer membrane that were found to be closely packed porin molecules. CM15 was introduced into the imaging fluid during continuous imaging at a final concentration of 20ug/mL, as indicated by the inset graph (Membrane Surface Roughness vs. Time). At ~96s after the addition of CM15, the cell membrane is observed to increase in roughness accompanied by the disappearance of the ordered membrane surface structures. Membrane protrusions start to form, consistent with micellization, followed by the appearance of "pore-like" lesions ~15nm in diameter. Once the effects of CM15 were initially observed, the subsequent changes in the cell surface occurred fairly rapidly over ~224s, at which point imaging of the cell surface becomes unstable.