Light-sheet fluorescence microscopy (LSFM) is a state-of-the-art imaging method to address a wide variety of biological questions. By decoupling the illumination and detection optical pathways, LSFM allows for a novel illumination strategy that optimizes the photon detection efficiency by only illumining the sample plane of interest. Since fluorescence detection is preformed along a different optical axis, this typically requires two (or more) objectives in an orthogonal orientation in order to maximize detection and minimize fluorescence from out of focus light. This axial confinement of the illumination beam results in LSFM having an extremely low rate of photobleaching and phototoxicity, allowing for long-term imaging for hours, days and even weeks. This technology opens the door for many researchers to preform high-speed 3D imaging of large and delicate sample while still being able observe fast subcellular processes and interactions within the organ, organoid or organism context.
In this webinar, we will introduce LSFM, highlight each of the different light sheet geometries Luxendo offers and show how LSFM can be adapted to every day imaging applications.