Microscopy in Biology and Medicine

To detect individual chemical substances

To detect individual chemical substances in a biological tissue and to visualize their distribution by visual light microscopy usually it is necessary to stain these substances using molecular colorants.

In contrast the IR-spectroscopic analysis does not require any staining of the molecular classes of interest. In fact they can be identified by the vibrational modes of their characteristic functional groups. As the signal intensities are proportional to the concentrations of the absorbing molecules the IR-measurement also shows the relative distribution of several molecular classes inside the analyzed area.

Animal and plant tissues are usually measured in transmission. For the FT-IR-microanalysis of biomedical materials like stents, implants or protheses also the other available measurement modes are applied.

Using microscopic FT-IR-imaging (HYPERION 3000) larger sample areas can be measured very fast and with high lateral resolution.

Typical Applications in Biology and Medicine are:

  • Analysis of the distribution of biochemical components (proteins, lipids, sugars, etc.) inside a tissue section (animal or plant material)
  • Characterization and classification of tissues
  • Identification and localization of amyloid protein plaques in tissues
  • Differentiation of tumors
  • Diagnostics of diseases by pattern recognition