Light-Sheet Microscopy: Seeing Life from a Different Angle
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Light-sheet fluorescence microscopy is opening the door for many researchers to preform high-speed 3D imaging of large and delicate samples while still being able observe fast subcellular processes and interactions within the organ, organoid or organism context. Register for this webinar to gain insights into the basics of light-sheet microscopy and the critical details that produce the best light-sheet imaging results.
Light-sheet fluorescence microscopy (LSFM) is a state-of-the-art imaging method to address a wide variety of biological questions. By decoupling the illumination and detection optical pathways, LSFM allows for a novel illumination strategy that optimizes the photon detection efficiency by only illumining the sample plane of interest. Since fluorescence detection is preformed along a different optical axis, this typically requires two (or more) objectives in an orthogonal orientation in order to maximize detection and minimize fluorescence from out of focus light. This axial confinement of the illumination beam results in LSFM having an extremely low rate of photobleaching and phototoxicity, allowing for long-term imaging for hours, days and even weeks. This technology opens the door for many researchers to preform high-speed 3D imaging of large and delicate sample while still being able observe fast subcellular processes and interactions within the organ, organoid or organism context.
In this webinar, we will introduce LSFM, highlight each of the different light sheet geometries Luxendo offers and show how LSFM can be adapted to every day imaging applications.
Who Should Attend
This webinar is geared towards researchers that have experience with high-end imaging, yet invites a broad enough conversation to interest those with basic microscopy knowledge. General microscopists will also benefit from attending. The broadcast will be of specific interest to researchers who:
- Do live-cell imaging
- Perform long-term time lapses
- Have photosensitive samples
- Express interest in new imaging technology
Key Topics Covered
- Introduction to Light-sheet Microscopy (LSFM)
- Comparison of imaging techniques
- Variations of light-sheet and their applications
- Sample preparation is everything!