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rapifleX - Brukers market-leading MALDI system

The rapifleX is the most advanced MALDI TOF/TOF system on the market today and it is also the most adaptable.


Whether you are looking at top-down sequencing for Biotherapeutics and Biosimilars, glycan structure analysis and confirmation, and localization or disulfide/scrambling/ trisulfide bond determination, the rapifleX is the best instrument for the job. 


The system combines state-of-the-art technology with advanced informatics into a system that gives you speed, robustness, versatility and confidence.

MALDI-TOF/TOF for every application and every market

Bruker has a long history of helping life scientists meet their toughest laboratory challenges. We provide the innovative MALDI TOF and MALDI TOF/TOF mass spectrometry systems that you rely on to accelerate your research.
 
The rapifleX is the latest example of Bruker’s dedication to developing and delivering innovative MALDI-TOF analysis technologies.

Now you can make the best decision in the shortest amount of time. In summary, the new Bruker rapifleX MALDI-TOF system represents a paradigm shift in terms of productivity, cost of ownership and ease of use.

The Bruker rapifleX platform offers a complete characterization system with unprecedented speed of analysis and performance.

The Bruker rapifleX platform offers a complete characterization system with unprecedented speed of analysis and performance.
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Dr. Julian D. Langer, MPI of Biophysics and MPI for Brain Research, Frankfurt am Main, Germany, about the rapifleX TOF/TOF´

"In our lab, with its high MS2 isolation efficiency, resolution and mass accuracy at high m/z values, the rapifleX TOF/TOF has been instrumental in identifying and characterizing new subunits of membrane protein complexes, including prokaryotic respiratory chain complexes and antibiotic drug targets."

The first MALDI TOF/TOF that adapts to your need

Our passion is mass spectrometry that you need. With the rapifleX series our engineers and developers have created the first MALDI–TOF system that now adapts to the analytical question at hand - automatically.

 

With speed in mind, we redesigned the heart of the rapifleX MALDI Imaging solution to work up to 20 times faster than traditional MALDI-TOF systems to meet the increasing requirements of tissue imaging or high throughput primary screening. 

 

The adaptable ion optics and class leading laser technology provide the best data quality, regardless of the acquisition mode or mass range.

The whole is more than the sum of its parts

The unprecedented 10 kHz speed and ion source robustness, the wide dynamic range, higher specificity and resolution, all contribute to the detailed characterization of biologically and clinically relevant lipids, peptides and proteins, raising customer MALDI-TOF/TOF expectations for applications such as:

In-depth protein characterization and imaging of tissues and cell cultures.

Most robust, yet easy to access ion source. All sensitive surfaces are in safe distance to the source of possible contaminations. When necessary, the capsular like ion source can be easily removed  and just rinsed with solvent. The rapifleX stands uninter
Most robust, yet easy to access ion source. All sensitive surfaces are in safe distance to the source of possible contaminations. When necessary, the capsular like ion source can be easily removed and just rinsed with solvent. The rapifleX stands uninterrupted primary screening with 2 Mio samples. (click to enlarge)
rapifleX: 25 years of knowledge and experience made solid.
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  • Speed - 10 kHz scanning smartbeam 3D laser for highest throughput – 5-20x increase in speed
  • Robustness - User removable ion source for easy cleaning, designed for maximum uptime in 24/7 operations
  • Confidence
    - Ion optics adapt to application
    - Three-stage reflector: No compromise, maximum resolution for full molecular weight range
    - Removable MS/MS ion optics for maximized sensitivity for MS analysis
    - MS/MS mass range for large peptides such as glycopeptides, di/trisulfide peptides and difficult non-tryptic peptides
    - Precursor ion selector resolution increased to 1000 –  for high selectivity in complex mixtures.
    - Supreme precursor ion selection for confidence in MS/MS data quality
    - 10 bit digitizer for high dynamic range

MALDI-TOF/TOF for every application and every market

The rapifleX MALDI tissuetyper is the complete solution for MALDI imaging.

  • Fastest acquisition with up to 40 pixel/second
  • Superior image quality
  • Integration of histology
  • Comprehensive software for the interpretation of large tissue-based biomarker studies

Identify Biomarkers directly from tissue

Even from complex  matrices, high quality MS/MS spectra can be acquired and identified.


The imageIDtm workflow combines tryptic digest on tissue with imaging and LC-MS for biomarker identification with confidence.

Accelerating Drug Discovery and Screening

The new rapifleX MALDI PharmaPulse is the first mass spectrometer to offer the speed, specificity and robustness required for large primary screens in drug discovery:

  • The integrated automation solution can process 1536 target plates in less than 8 minutes ~ 0.3sec/sample
  • Up to 100 times less solvent than multi-plexed LC/SPE-MS systems
  • No need for SPE cartridges or HPLC columns
  • Label-free detection accelerates and simplifies assay development and avoids interferences from artifacts
  • Extremely low sample volumes required for detection (typically ~1μL) enables single well kinetic readouts which can reduce the total number of assays required

Verification by high sequence coverage

Top-down sequencing spectrum of the c-ring subunit of M. phlei ATP synthase containing a mutation which causes antibiotics resistance with an overall sequence coverage of 99%.

Modification and mutation sites are detected unambiguously.

Confident Characterization of Biotherapeutics

Automated disulfide bond determination via LC-MALDI analysis (left) followed by manual quantification of trisulfide forms. Quantitation of trisulfide forms in human IgG1 monoclonal antibodies: the 10 bit digitizer allows for quantitation with high dynamic range down to the relevant 0.1-1% range.

Automated disulfide bond determination via LC-MALDI analysis
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Proteins in sagittal rat brain section measured at 30μm pixel size, ~150000 pixel.
Proteins in sagittal rat brain section measured at 30μm pixel size, ~150000 pixel. (click to enlarge)
Tryptic peptide directly identified from tissue
Tryptic peptide directly identified from tissue (click to enlarge)
Full 1536 plate reading in less than 8 minutes
Full 1536 plate reading in less than 8 minutes (click to enlarge)
Top-down sequencing spectrum
Top-down sequencing spectrum (click to enlarge)

Bruker Daltonics services are performed by factory trained and certified engineers. DIN EN ISO 9001:2000 requirements are fulfilled

Available services include:

  • Help desk service for a faster access to our support team, to your service order follow up and for general information
  • Planned maintenance, according to our requirement
  • Telephone support from highly-skilled troubleshooting professionals, to isolate and resolve hard- and software problems
  • Web-based remote instrument service for service diagnosis and applications support
  • Customer on-site repair and maintenance service
  • Spare parts availability typically over night or within a few working days worldwide
  • Compliance services for installation qualification, operational qualification / performance verification
  • Site planning and relocation
  • Advise to improve labs efficiency (consumable, method development & how to run the complete solution)

For Research Use Only. Not for Use in Clinical Diagnostic Procedures.