The metabolome is the final manifestation of biochemical pathways. It shows an extremely large variety of structural classes as it includes the complete set of metabolites of all cellular processes.
Temporal and spatial changes in the metabolite composition reflect the outcome (phenotype) of interactions at the genomic, transcriptomic and proteomic level. Therefore, studying the phenome is a cornerstone to gaining deeper insights related to e.g. diseases, therapeutic interventions or environmental influences.
The aim of untargeted metabolomics is the global profiling of small molecule biomarkers that are characteristic for a particular physiological state. A major requirement is to quickly pinpoint and identify those compounds that change as a result of perturbation or disease.
Together with Bruker Compass® TASQ™ 2.0 software, both targeted validation and untargeted metabolomics experiments are supported. Double your level of insight by performing biochemical pathway-driven targeted analysis on the same data set.
MetaboScape 4.0 is able to process complementary data from Bruker's LC-QTOF-MS/MS, GC-APCI-QTOF, LC-TIMS-MS and MRMS systems:
- T-ReX 2D: FIA-MRMS data for ‘MetaboTyping’
- T-ReX 3D: LC-MS data e.g. from the Phenomics Workhorse
- T-ReX 4D: LC-TIMS-MS data from timsTOF
Using the new releases of the Bruker HMDB Metabolite Library 2.0, the MetaboBASE Personal Library 2.0 or the MetaboBASE Plant Library, confidence in the de-replication of known compounds can be boosted.
Learn more about the libraries
As many as five important criteria for data quality are automatically matched and displayed visually using the “Annotation Quality Scoring” icon:
