After adjustment of the 3D orientation in Dataviewer, the different phases of the beating heart can visualized. Isolating the left ventricle by drawing an ROI and thresholding the blood, allows us to calculate the blood volume at every phase, and from this the ejection fraction. The same ROI can be loaded on every dataset (corresponding to the different bins) because all bins are originating from the same animal. The thresholding and possible additional image processing steps can easily be transferred to the custom processing tab in CTAn. Once the protocol or ‘task list’ is generated, we can use BatMan to load all datasets and fully automate the analysis process. For visualization purposes, adding a ‘3D model’ in the task list, multiple 3D surface rendering models will be generated corresponding to the change in blood volume of the left ventricle. A step by step explanation can be found in the method note MN079.